紫外光谱法在芡实种子品种和产地鉴别中的应用

Application of ultraviolet spectrometry in the identification in variety and origin for gordon euryale seeds

以两种品种（天长刺芡和苏芡）和产地（天长和湖北刺芡）芡实为材料，分别制备4种提取液（乙酸乙酯、无水乙醇、50％乙醇、70．80℃热水），分析其紫外光谱吸收强度的差异性。同时，对芡实种子结构特性（百粒重、种子及种仁直径、种壳厚度）和功能组分含量（总酚、原花青素及黄酮）差异性进行分析。究发现，除不同产地芡实种壳乙酸乙酯和无水乙醇提取物之外，两种品种（产地）芡实种壳提取物最大吸光值之间均存在极显著差异（p≤0.01），其种子和种仁直径、种壳厚度、种仁原花青素、种壳总黄酮和总酚含量差异极显著（p≤0．01），结果表明，紫外光谱分析在芡实品种和产地方面有一定区分度，为芡实品种和产地鉴别提供了一种思路。

Four kinds of extracts （acetic ether, absolute ethyl alcohol, 50% alcohol, 70-80℃ hot water） were prepared with two kinds of variety（north gordon euryale and South gordon euryale from Tianchang） and origin （north gordon euryale from Tianchang and South gordon euryale from Hubei） gordon euryales as the experimental materials ,and then the variances of the ultraviolet absorption spectrum intensity were analysed. Simultaneously,the variances between structural property（hundred-grain weight,diameters of seeds and seed kernel,thickness of seed shell） and active ingredients（total phenol,proanthocyanidins and flavone） of gordon euryale seeds were analyzed. It was discovered in the study that there were all highly significant differences in the maximum peak absorption values of extracts which comed from two kinds of variety and origin gordon euryale seed shell （p≤0.01） in addition to the acetic ether and alcohol extracts of gordon euryale seed shell from different origin ,and the variance was extremely remarkable between the diameters of seeds and kernel, thickness of seed shell,proanthocyanidins in kernel and the content of total flavonoids and total phenol in the seed shell（p≤0.01 ）. The search results showed that ultraviolet spectral analysis could have certain discrimination in the variety and origin of gordon euryale,which provided a mind for the identification of gordon euryale variety and origin.