萌发对芸豆酚类物质及抗氧化活性的影响

Effect of germination on the content of polyphenols and antioxidation activity of kidney beans(Phaseolus vulgarisl L.)

研究了萌发过程对黑芸豆、奶花芸豆、紫花芸豆中多酚、黄酮含量及抗氧活性的影响，并对多酚、黄酮含量及抗氧化能力做了相关性分析。结果表明：三种芸豆萌发（0—6d）后多酚、黄酮含量及抗氧化活性较未萌发的均显著降低（p〈0．05）。且浸泡损失的多酚、黄酮高于萌发期间；黑芸豆萌发6d后多酚、黄酮含量可能由于内切酶的作用开始增加。萌发期间，三种芸豆的DPPH·清除能力（DPPH）、ABIS＋·清除能力（ABTS）、铁离子还原抗氧化能力（FRAP）、氧化自由基吸收能力（ORAC）均有降低趋势。多酚、黄酮含量与抗氧化能力极显著相关（p〈0．01），且多酚与ABTS值的相关性最高（r=0．955）。此外，黑芸豆的抗氧化能力萌发4d后开始增加，萌发6d时黑芸豆和奶花芸豆的ORAC值高于0d合适的萌发条件能更好的保留芸豆的生物活性物质。

The effect of germination on the content of polyphenols and flavonoids and antioxidant activity of black beans ,speckled kidney beans ,and purple beans was studied. The correlation between phenolic content, flavonoid content and antioxidant activity was analyzed. It was found that the content of phenolics and flavonoids in all three germinated beans（0-6 d） significantly lower than in raw beans（p〈0.05）. The loss of phenolics and flavonoids during soaking stage was higher than that during germination stage among all three beans. Interestingly,after germination for 6 days,content of phenolics and flavonoids in black bean began to increase,implying phenolics release caused by endo-enzymes. Generally,the antioxidant activity determined with DPPH. radical scavenging capacity（DPPH）,ABTS＋. radical scavenging capacity （ABTS）,ferric reducing antioxidant power （FRAP） or oxygen radical absorption capacity （ORAC） all showed decreasing trends during germination process,which was highly correlated with content of phenolics and flavonoids in the beans. The most correlated indices with r of 0.955 in this study was content of phenolics and the ABTS values. Research also showed that the antioxidant activity of black beans increased after 4 day germination,and the ORAC value of black beans and speckled kidney beans after 6 day germination were even higher than those of 0 d. In conclusion, better biologically active substance could be obtained for different beans under suitable conditions.