红曲黄色素的分离纯化与表征

The separation and purification of monascus yellow pigment and characterization

采用硅胶柱层析法对菌体内醇溶性红曲黄色素进行分离、纯化,并对纯化后的红曲黄色素结构及纯度进行液相质谱鉴定表征,得到柱层析最佳洗脱条件:洗脱剂配比正己烷∶乙酸乙酯(v/v)=1∶8,上样量1.5 m L,洗脱流速50 m L/h。分离纯化所得的红曲色素经液相质谱检测含红斑胺素、红曲素、红斑素三种色素组分,采用相对定量法对分离所得的红曲黄色素组分进行纯度表征,结果显示其纯化保存率达85%。

Silica gel column chromatography was used to separate monascus yellow pigment in mycelia,and the structure and purity of monascus yellow pigment were identified by HPLC-MS. The separation conditions were researched and determined. The best separation conditions were as follows ：the mixture of n-hexane and ethylacetate（volume ratio of 1：8）,the best sample was 1.5 mL,the best elution agents velocity was 50 mL/h. Rubropunctamine,Rubropunctatin and Monascine were detected by HPLC-MS after separation of the sample. The relative quantitative method was used to verify the yellow pigment component of purified amounted to 85%.