摘要
采用硅胶柱层析法对菌体内醇溶性红曲黄色素进行分离、纯化,并对纯化后的红曲黄色素结构及纯度进行液相质谱鉴定表征,得到柱层析最佳洗脱条件:洗脱剂配比正己烷∶乙酸乙酯(v/v)=1∶8,上样量1.5 m L,洗脱流速50 m L/h。分离纯化所得的红曲色素经液相质谱检测含红斑胺素、红曲素、红斑素三种色素组分,采用相对定量法对分离所得的红曲黄色素组分进行纯度表征,结果显示其纯化保存率达85%。
Silica gel column chromatography was used to separate monascus yellow pigment in mycelia,and the structure and purity of monascus yellow pigment were identified by HPLC-MS. The separation conditions were researched and determined. The best separation conditions were as follows :the mixture of n-hexane and ethylacetate(volume ratio of 1:8),the best sample was 1.5 mL,the best elution agents velocity was 50 mL/h. Rubropunctamine,Rubropunctatin and Monascine were detected by HPLC-MS after separation of the sample. The relative quantitative method was used to verify the yellow pigment component of purified amounted to 85%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第16期308-312,323,共6页
Science and Technology of Food Industry
基金
国家自然科学基金青年基金项目(31401669
31301411)
北京市科技计划项目
北京市属高等学校食品科学创新团队项目(IDHT20130506)
关键词
红曲黄色素
硅胶柱层析
分离纯化
液相质谱
monascus yellow pigment
Silica gel column chromatography
separation and purification
HPLC-MS