角鲨烯对小鼠急性酒精性肝损伤的保护作用

Protective effects of squalene on acute alcohol-induced liver injury in mice

目的:研究角鲨烯对急性酒精性肝损伤的保护作用。方法:60只ICR小鼠随机分为正常组,模型组,阳性组,角鲨烯高、中、低剂量组。正常组和模型组灌胃等体积花生油,阳性组灌胃葵花牌护肝片350 mg/kg;角鲨烯高、中、低剂量组分别灌胃角鲨烯500、250、125 mg/kg,每日1次,30 d后制备急性酒精性肝损伤模型。模型制备16 h后检测肝脏系数,血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和甘油三酯(TG)水平及肝脏丙二醛(MDA)和还原型谷胱甘肽(GSH)含量。结果:角鲨烯可明显降低血清ALT、AST、TG水平,降低肝组织MDA含量,并提高肝组织GSH含量,且呈明显的剂量依赖性,大剂量还能明显降低肝损伤小鼠的肝脏系数。结论:角鲨烯对小鼠急性酒精性肝损伤具有明显的保护作用,其机制可能与其抗氧化作用有关。

Objective：To study the protective effects Methods：Sixty ICR mice were randomly divided of squalene on acute alcohol-induced liver injury in mice nto normal group,model group,positive group,and high midium,low dose group of squalene. The mice of normal group and model group were given peanut oil, positive group were given 350 mg/kg huganpian,and high,midium,low dose group of squalene were given 500 mg/kg,250 rag/kg, 125 mg/kg squalene. After 30 days ethanol were given to all groups except the normal. The level of glutamic-pyruvic transaminase （ALT）, glutamic oxaloacetictransaminase （AST）,triglyceride （TG） in serum and the content of malondialdehyde （MDA）, reduced glutathione （GSH） in liver were detected. Results ： Squaiene could reduce the level of ALT,AST,TG and MDA,and improve the content of GSH. Conclusion： Squalene could protect against acute alcoholic hepatic injury in mice, possibly due to its antioxidant effect.