硫化氢对脓毒症大鼠电解质和肾功能的影响

The effects of hydroge n sulfide on the electrolytes and renal function in sepsis rast

目的：探讨硫化氢（ H2S）对脓毒症大鼠电解质及肾功能的影响。方法92只雄性大鼠，其中20只随机分为正常大鼠组和盲肠结扎穿刺（CLP）6、12、24 h组（每组n＝5），72只随机分为假手术组、CLP＋生理盐水组（n＝18）、CLP＋硫氢化钠（NaHS）组（n＝18）、CLP＋炔丙基甘氨酸（PAG）组（n＝18）。术前1 h分别经腹腔注射生理盐水、NaHS和PAG。相应时间点处死后留取血浆标本，使用全自动生化分析仪检测血浆电解质和肌酐（ Cr）、尿素氮（ BUN）浓度。结果与正常大鼠组比较，CLP术后血浆Na ^＋浓度先增高再逐渐降低而后再次升高至正常水平；血浆Cl －浓度逐渐增高至6 h后呈时间依赖性降低；血浆K^＋、BUN和Cr浓度均呈时间依赖性升高；术后血浆Ca^2＋浓度明显降低。与CLP＋生理盐水组比较，CLP＋NaHS组术后血浆Na^＋、K^＋、Cl^－、Cr和BUN浓度均明显降低，Ca^2＋浓度变化差异无统计学意义。 CLP＋PAG组术后各时间点血浆Na^＋、Cl^ －、K ^＋、BUN和Cr浓度均明显升高，血浆Ca ^2＋浓度变化差异无统计学意义。结论脓毒症大鼠可引起电解质紊乱，早期即出现肾功能损伤并随病程时间延长而逐渐加重。 H2S参与脓毒症时电解质及肾功能调节。脓毒症时H2S可能通过影响肾功能而达到调节血浆电解质浓度的目的。

Objective To investigate the effects of hydrogen sulfide （ H2S ） on serum electrolytes and renal function in sepsis rats .Mtehods A total of 92 male Spargue-Dawley rats were used in the present study.Twenty rats were randomly divided into 5 groups： normal rats group, cecal ligation puncture（ CLP） 6 h, 12 h, 24 h group （ n=5/group） .Seventy-two rats randomly divided into sham operate group, CLP＋saline group （n=18）, CLP＋sodium hydrosulfide （NaHS） group （n=18） and CLP ＋propargylglycine （ PAG ） group （ n=18 ） .Rats were administrated with saline, NaHS or PAG respectively by peritoneal injection 1 h before operation.Rats were sacrificed at designated time and serum was collected to detect serum electrolytes, creatinine （ Cr ） and urea nitrogen （ BUN ） concentrations.Results Compared with normal rats, serum Na^＋level increased followed by a decline, then returned to normal level after CLP.Serum Cl-level increased after CLP till 6 h.After 6 h, serum Cl-level started to decline in a time-dependent manner.Serum K^＋, Cr and BUN levels increased in a time-dependent manner after CLP.Serum Ca^2＋level decreased at all time points after CLP.Compared with CLP＋saline group, serum Na^＋, Cl^-, K^＋, Cr and BUN levels were significantly decreased in CLP＋NaHS group.There was no effect on serum Ca^2＋level at all time points.At all time points in CLP＋PAG group, serum Na^＋, Cl^-, K^＋, Cr and BUN levels were significantly elevated and serum Ca^2＋level do not significantly changed.Conclusion Sepsis induce serum electrolytes disturbance. Renal function is injured at early stage of sepsis and deteriorates in a time-dependent manner.H2 S regulates electrolyte levels and renal function during sepsis and the effects on kidney may involve in regulation of serum electrolyte concentrations during sepsis.