摘要
目的观察螺旋藻多糖对慢性乙型肝炎患者外周血单个核细胞免疫功能的影响。方法运用MTT法和流式细胞术分别测定螺旋藻多糖对慢性乙型肝炎患者外周血单个核细胞增殖能力和细胞周期的影响,采用ELISA法检测外周血单个核细胞培养上清细胞因子IFN-γ、IL-2和IL-4浓度,同时用RT-PCR法检测外周血单个核细胞IFN-γm RNA的表达。结果螺旋藻多糖能增强慢性乙型肝炎患者外周血单个核细胞的增殖能力,能增强慢性乙型肝炎患者外周血单个核细胞分泌IFN-γ和IL-2,且能降低分泌IL-4。螺旋藻多糖能上调慢性乙型肝炎患者外周血单个核细胞IFN-γm RNA的表达。结论螺旋藻多糖对慢性乙型肝炎患者外周血单个核细胞的免疫功能具有调节作用。
Aim To investigate the immunoregulative action of polysaeehrldes of Spirulina platensis （PSP） on peripheral blood mononuclear cells （PBMCs） in pa- tients with chronic hepatitis B virus infection. Meth- ods Proliferation and cycle of PBMCs from patients with chronic hepatitis B virus infection were measured by MTT colorimetry and flow cytometry respectively. The concentration of IFN-γ, IL-2, IL-4 in supernatants of PBMCs were examined by ELISA. The mRNA ex- pression of IFN-γin PBMCs from patients with chronic hepatitis B virus infection was detected by RT-PCR. Results PSP could induce proliferation of PBMCs in patients with chronic hepatitis B virus infection. In ad- dition, PSP could increase the protein expression ofIFN-γ and IL-2 in supernatants of PBMCs in patients with chronic hepatitis B virus infection, and PSP could also enhance the mRNA expression of IFN-γof PBMCs in patients with chronic hepatitis B virus infection. However, PSP inhibited the protein expression of IL-4 of PBMCs in patients with chronic hepatitis B virus in- fection. Conclusion PSP can promote the immune function and plays an important immunoregulation function on patients with chronic hepatitis B virus in- fection.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2015年第8期1121-1125,共5页
Chinese Pharmacological Bulletin
基金
广东省社会发展领域科技计划项目(No 73043)
湛江市财政资金科技专项备用经费资助项目(No 2014C01032)
广东医学院面上项目(No M2012013)
