摘要
目的探讨丹皮酚(paeonol)对人舌癌TCA8113细胞生物学行为的影响。方法用MTT法检测不同浓度的丹皮酚对TCA8113细胞增殖的抑制的作用;用ELISA实验检测细胞血管内皮生长因子(VEGF)蛋白的表达水平。结果 MTT实验表明丹皮酚对TCA8113细胞的增殖有抑制作用,并随浓度增加抑制作用增强;ELISA实验提示丹皮酚抑制TCA8113细胞分泌VEGF蛋白。结论丹皮酚可以抑制TCA8113细胞增殖,抑制VEGF蛋白的分泌。
Objective It is to research the effect of paeonol on the biological behavior of human tongue carcinoma in TCA8113 cells. Methods MTT was used to detect the inhibition of paeonol on the proliferation of TCA8113 cells, with different concentration. Expression level of VEGF was detected by ELISA assay. Results MTT results showed that paeonol has inhibition effects on the proliferation of TCA8113 cell. The inhibition effects are enhanced with the increase of its concentration. ELISA assay indicates that paeonol has inhibited TCA8113 cells secrete VEGF. Conclusion The proliferation of TCA8113 ceils can be inhibited by paeonol, and paeonol can inhibi TCA8113 cells secrete VEGF.
出处
《中国继续医学教育》
2015年第20期200-201,共2页
China Continuing Medical Education