谷物膳食纤维对脂代谢紊乱小鼠肝脏脂毒性影响

Effects of cereal dietary fiber on lipid metabolism and lipotoxicity in mice fed with high fat / cholesterol diet

目的观察不同类型谷物膳食纤维(DF)对脂代谢紊乱小鼠血脂和肝脏脂毒性影响。方法 48只雄性C57BL/6J小鼠(6周龄)按体重随机分为4组:对照组、模型组、燕麦DF组、小麦DF组,连续干预24周,实验期间每周称体重并记录摄食量,每8周内眦静脉采血,进行血脂和血糖连续动态观察;干预结束后测定空腹血糖、血清总胆固醇(TC)、甘油三酯(TG)和游离脂肪酸(FFAs)及肝组织TG含量。结果与对照组比较,模型组小鼠体重[(37.69±2.66)g]、空腹血糖[(8.21±2.06)mmol/L]、血清TC、TG、FFAs水平[分别为(2.69±0.45)、(1.77±0.64)mmol/L、(2.12±0.38)m Eq/L]明显升高(P<0.05);与模型组比较,燕麦DF与小麦DF组小鼠体重[分别为(34.41±2.93)、(34.43±1.72)g]、血清TC、TG水平[分别为(2.03±0.44)、(2.22±0.50)与(1.64±0.43)、(1.39±0.41)mmol/L]均明显下降(P<0.05);燕麦DF组小鼠血清FFAs水平[(1.75±0.49)m Eq/L]明显降低(P<0.05)。结论谷物DF可以改善脂代谢紊乱小鼠血脂和肝脏脂毒性,燕麦DF改善血脂和肝脏脂毒性的作用优于小麦DF。

Objective To investigate effects of different cereal dietary fiber on lipid metabolism and lipotoxicity in mice fed with high fat/cholesterol diet. Methods Forty-eight male C57BL/6J mice were randomly divided into four groups according to body weight： a control group, high fat/cholesterol group, high fat/cholesterol ＋ oat dietary fiber group, and high fat/cholesterol ＋ wheat dietary fiber group. During the experiment, body weight and food intakes were recorded weekly. The blood samples were collected from angular vein every 8 weeks for measurements of serum total cholesterol （TC）, triglyceride （TG）, and blood glucose. After 24 weeks, the final fasting blood glucose, serum TC, TG, free fatty acids （ FFAs）, and liver homogenate TG were measured. Results Compared with those of the control group, the body weight（37.69 ±2. 66 g） ,fasting blood glucose（8.21 ±2. 06mmol/L） ,serum TC（2. 69 ±0. 45 mmol/L） ,TG （ 1.77 ±0. 64 mmol/L） and FFAs（2. 12 ±0. 38 mEq/L） of high fat/cholesterol diet group were significantly increased （P 〈0. 05 for all）. Compared with those of the high fat/cholesterol diet group,the body weight（34. 41 ± 2.93 g and 34.43 ± 1.72 g） ,serum TC（2. 03 ± 0.44 and 2. 22 ± 0. 50 mmol/L）, and serum TG（ 1.64 ± 0. 43 and 1.39 ± 0. 41 mmol/L） were significantly decreased in high fat/cholesterol ＋ oat dietary fiber group and high fat/cholesterol ＋ wheat dietary fiber group（ P 〈 0. 05 for all）. Compared with that of the high fat/cholesterol diet group,the serum FFAs （1.75±0. 49 mEq/L） was significantly decreased in the oat dietary fiber group （P 〈0. 05 ）. Conclusion Cereal dietary fiber can improve lipid metabolism and lipotoxicity in mice fed with high fat/cholesterol diet and the effects of oat dietary fiber on blood lipid and liver lipotoxicity is better than those of wheat dietary fiber.