大豆异黄酮对老龄雌性大鼠血清性激素水平影响

Eeffect of soy isoflavones on serum sex hormones in aged female rats

目的观察大豆异黄酮对老龄雌性大鼠卵巢及血清中主要性激素水平影响。方法选用20月龄老龄雌性大鼠50只,按体重随机分为5组,每组10只,分别为空白对照组,大豆异黄酮低、中、高剂量组(100、200、300mg/kg)、雌激素组(己烯雌酚0.6 mg/kg),连续给予8周。采用酶联免疫吸附实验测定各组大鼠卵巢及血清中雌二醇、孕酮、睾酮、黄体生成素(LH)、促卵泡生成素(FSH)、性激素结合球蛋白(SHBG)水平。结果与对照组比较,高剂量大豆异黄酮组大鼠卵巢/体比(0.031±0.008)明显降低(P<0.05),血清中雌二醇含量[(5.177±0.453)μg/L]明显升高,LH含量[(3.225±0.405)ng/L]、FSH含量[(1.536±0.186)IU/L]降低(P<0.05);与对照组比较,大豆异黄酮组大鼠血清中孕酮、睾酮、SHBG水平无明显变化(P>0.05)。结论大豆异黄酮可通过调节老龄雌性大鼠血清中雌二醇、LH、FSH水平缓解卵巢功能衰退。

Objective To observe the effect of soy isoflavones on main serum sex hormones and ovaries in aged fe- male rats. Methods Fifty 20-month-old female rats were randomly divided into five groups：control group （with oral ga- vage of normal saline） ,low-, moderate-, and high-dose group（ with oral gavage of soy isoflavones of 100,200, and 300 mg · kg- 1 ） , estrogen group（ with oral gavage of diethylstilbestrol of 0. 6 mg · kg- 1 ）. After 8 weeks＇ of treatments, the levels of estradiol, progesterone, testosterone, luteinizing hormone （ LH）, follicle stimulating hormone （ FSH）, and sex hor- mone binding globulin（SHBG） in serum of the rats were detected with enzyme-linked immunosorbent assay （ELISA）. Results Compared with those of the control group, the ratio of ovary/body （ 0. 031 ±0. 008 ）, serum LH （ 3. 225 ±0. 405 ng/L） and FSH（ 1. 536 ±0. 186 IU/L） decreased（P 〈0. 05 for all） ,whereas the serum estradiol（5. 177 ±0. 453 μg/L） increased（P 〈0. 05） for the rats of high-dose soy isoflavones group,but no significant differences in uterus/body ratio, serum progesterone, testosterone, and SHBG were observed（ P 〉 0. 05 ）in the rats of soy isoflavones groups. Conclu- sion Soy isoflavone could regulate serum levels of main sex hormones and alleviate the decline of ovarian function in aged female rats.