高功率微波辐射对家兔心肌形态和心肌酶影响

Effect of high power microwave on myocardial morphology and enzymes in rabbits

目的探讨高功率微波(HPM)辐射对家兔心肌形态结构和心肌酶的影响。方法新西兰家兔24只,雌雄各半,随机分为对照组和微波辐射高、中、低密度组,辐射功率分别为200、150、100 m W/cm2,每组6只,辐射20 min,辐射后6 h,股动脉取血,检测心肌酶活性,冰浴下立即取心肌心尖部组织,制作常规光镜标本并观察。结果微波辐射后6 h时,与对照组比较,微波辐射低密度组家兔血清肌酸激酶同工酶、肌酸激酶和乳酸脱氢酶活性[分别为(619.5±268.3)、(841.7±131.4)和(376.5±149.6)U/L]明显升高,差异有统计学意义(P<0.05),呈剂量效应关系;光镜下大部分心肌细胞胞浆疏松淡染,部分心肌纤维断裂,心肌间质内血管扩张充血、伴有炎性细胞浸润,病变程度与辐射功率呈正相关。结论高功率微波辐射对家兔心肌具有损伤作用,损伤效应包括心肌结构与心肌酶改变。

Objective To investigate the effect of high power microwave （HPM） on myocardial morphology and myocardial enzymes in rabbits. Methods Twenty-four adult rabbits （ half male and half female） were randomly divided into four groups：a control group ,high- （200 mW/cm2）, moderate- （150 mW/cm2）, and low-intensity（ 100 mW/cm2） radiation groups with the HPM exposure of 20 minutes. All experimental rabbits were sacrificed 6 hours after the treat- ment and their femoral artery blood samples were collected; the activities of myocardial enzymes were examined. The heart apex fresh tissues were taken immediately under ice bath and the pathological changes of the tissues were observed with light microscopy. Results Compared to those of the control group, the activities of serum creatine phosphokinase isoenzyme（CK-MB） （619. 5 ＋268.3 U/L） ,creatine phosphokinase（CK） （841.7 ± 131.4 U/L） ,and lactate dehyroge- nase（LDH） （376. 5 ± 149. 6 U/L） increased significantly in the rabbits exposed to low-intensity（ 100 mW/cm2 ） radia- tion （ P 〈 0. 05 for all）. The pathological changes of the myocardial tissues were observed, with loose and light staining of cytoplasm, fracture of muscle fiber, angiectasis hyperemia of myocardial interstitiurn, and inflammatory cell infiltration. The pathological changes were positively correlated with the intensity of HPM exposure. Conclusion High power micro- wave radiation had damage effect on myocardial morphology and enzymes in rabbits.