摘要
[目的]探讨贝伐珠单抗对结直肠癌细胞血管内皮生长因子(VEGF)相关受体的影响。[方法]RT-PCR、Western blotting检测不同浓度贝伐珠单抗对结直肠癌肿瘤细胞VEGF相关受体表达水平的影响。[结果]与对照组相比,贝伐珠单抗药物组结直肠癌细胞的VEGF表达水平均明显下降(P<0.05);VEGFR1和VEGFR2表达水均明显上调(P<0.05)。[结论]贝伐珠单抗可引起了结直肠癌细胞VEGFR1和VEGFR2表达上调。
[Purpose] To investigate the effect of bevacizumab on vascular endothelial growth factor（VEGF） receptors on colorectal carcinoma cells. [Methods] The changes of VEGF receptors expression on colorectal carcinoma cell at different concentrations of bevacizumab were detected by RT-PCR and western blotting. [Results] Compared with the controls,the expressions of VEGF were both decreased on bevacizumab-induced colorectal carcinoma cells（P〈0.05）. Besides the expressions of VEGFR1 and VEGFR2 were both up-regulated on bevacizumab-induced colorectal carcinoma cells（P〈0.05）. [Conclusions] Bevacizumab induced the up-regulation of VEGFR1 and VEGFR2 on colorectal carcinoma cells.
出处
《中国肿瘤》
CAS
2015年第8期708-714,共7页
China Cancer
基金
北京科委首都医疗特色项目(Z131107002213040)~~
