苍菊清肝降脂方对大鼠脂肪肝保护作用的研究

Effect of Cangju Qinggan Jiangzhi recipe on fatty liver in rats

[目的]研究苍菊清肝降脂方对大鼠脂肪肝的保护作用。[方法]雄性Wistar大鼠40只,随机分正常组、模型组、低剂量组、高剂量组。模型组、低剂量和高剂量组以40%CCl4橄榄油溶液1ml/kg体质量腹腔注射,2次/周,共4周。模型组、低剂量和高剂量组在第1、2周给予高脂低蛋白饲料,第3、4周予纯玉米粉饲料。自造模之日起,按照1ml/100g大鼠体重给予低剂量和高剂量中药灌胃。实验结束后大鼠经下腔静脉采血并分离血清,统一于肝脏左叶切取组织,于10%中性福尔马林固定。检测血清ALT、AST、GGT活性,肝组织行HE染色。[结果]模型组大鼠血清ALT、AST和GGT均较正常组显著增高(P<0.01);经苍菊清肝降脂方中药干预,ALT、AST和GGT均显著下降(P<0.05),其中高剂量组ALT和AST下降幅度均优于低剂量组(P<0.05),而低剂量组和高剂量组GGT下降幅度无统计学意义(P>0.05)。HE染色可见模型组大鼠小叶结构紊乱,肝细胞胞质疏松,肝细胞变性,胞浆内见大量脂肪空泡。苍菊清肝降脂方干预组可见大鼠肝细胞损伤及脂肪变性程度明显减轻,肝小叶结构比较清晰,肝细胞胞浆内脂肪空泡与模型组相比有显著差异,尤其以高剂量组改善程度更为明显。[结论]苍菊清肝降脂方对大鼠脂肪肝具有良好的保护作用。

[Objective]To study the protective effect of Cangju Qinggan Jiangzhi recipe on rats with fatty liver.[Methods]Forty male Wistar rats were randomly divided into normal,model,low-dose and high-dose group.Rats in model,low-dose and high-dose group were intraperitoneal injected with 40% CCl4 olive oil solution by 1ml/kg body weight,2times/week for 4weeks.Rats in model,low-dose and high-dose group were given high-fat and low-protein diet during the first 2 weeks,and pure corn diet during the rest weeks.From the beginning of experiment,Cangju Qinggan Jiangzhi recipe was given orally by 1ml/100 g body weight with low-and high-dose.At the end of experiment,blood was collected through inferior vena cava and sera were separated.Rat liver left lobe was collected and fixed in 10% neutral formalin.Serum ALT,AST,GGT activity were determined,liver tissue were stained with HE.[Results]Serum ALT,AST and GGT in model group were significantly higher than in control group（P〈0.01）.ALT,AST and GGT in intervention groups were significantly decreased（P〈0.05）;ALT and AST in the high dose group decrease were better than in the low dose group（P〈0.05）,while GGT was not statistically significant（P〉0.05）.In model group,HE staining showed that lobular structure disorder,hepatic cells cytoplasm rarefaction,degeneration of liver cells,fat vacuoles in cytoplasm.In intervention groups,the damage in rat liver cells and fatty degeneration significantly reduced,the structure of hepatic lobules was clear,the cytoplasm of liver cells of fat vacuoles compared with the model group had significant differences,improved more obviously especially in the high dose group.[Conclusion]Cangju Qinggan Jiangzhi recipe has good protective effect on fatty liver in rats.