芪黄煎剂对大鼠胃切除后肠淋巴细胞归巢过程及肠黏膜屏障的影响

Effects of Qihuang Decoction on intestinal lymphocytes homing process of gut mucosa after gastrectomy in rats

[目的]探讨芪黄煎剂对大鼠胃切除后肠淋巴细胞归巢过程的影响。[方法]60只SD大鼠随机分为假手术组、对照组、芪黄煎剂组。假手术组仅给予腹部正中切开后缝合,不行胃切除,不给予肠内营养和芪黄煎剂;对照组行胃切除手术后给予肠内营养制剂;芪黄煎剂组行胃切除手术后给予肠内营养制剂和芪黄煎剂,疗程一周。疗程结束后分别观察:淋巴细胞归巢数量;淋巴液中T淋巴细胞(αβTCR-CD3+T细胞及其亚群CD4+、CD8+T细胞);PP结IgA+B细胞。[结果]与假手术组比较,对照组中pp结、肠系膜淋巴结中归巢淋巴细胞百分比显著下降(P<0.01);与对照组比较,芪黄煎剂组pp结、肠系膜淋巴结中归巢淋巴细胞百分比高于对照组(P<0.05或P<0.01)。与假手术组比较,对照组和芪黄煎剂组CD3+T细胞比例下降(P<0.01或P<0.05),芪黄煎剂组CD3+T细胞比例较对照组相比升高(P<0.01);与假手术组相比,对照组和芪黄煎剂组CD4+T细胞比例下降(P<0.01或P<0.05),芪黄煎剂组同对照组相比,差异无统计学意义(P>0.05);与假手术组比较,对照组CD8+T细胞比例下降(P<0.05),芪黄煎剂组同假手术组及对照组比较,均未见明显差异(P>0.05)。与假手术组比较,对照组和芪黄煎剂组pp节中IgA+B细胞比例显著下降(P<0.01和P<0.05);芪黄煎剂组中IgA+B细胞比例高于对照组(P<0.01)。[结论]中药芪黄煎剂可通过促进胃切除手术后肠淋巴细胞归巢,从而保护肠黏膜免疫屏障,防治细菌移位发生。

[Objective]To observe the effects of Qihuang decoction on intestinal lymphocytes homing process of gut mucosa after gastrectomy in rats.[Methods]Sixty rats were randomly divided into three groups,the sham operation group,the control group and the Qihuang decoction group.The sham operation group was only sutured after abdominal midline incision,without gastrectomy,without giving enteral nutrition and Qihuang decoction.The control group was given enteral nutrition after gastrectomy.The Qihuang decoction group was given enteral nutrition and Qihuang decoction after gastrectomy.The course of treatment was a week for every group.After the course of treatment,the number of lymphocyte homing,T lymphocytes in lymph（αβTCR-CD3＋T cells and the subsets of CD4＋,CD8＋T cells）and IgA＋B cell in Peyer’s patches were observed respectively.[Results]Compared with the sham operation group,the lymphocyte homing percentage of Peyer’s patches and mesenteric lymph nodes in the control group were significantlydecrease（P〈0.01）.Compared with the sham operation group,the lymphocyte homing percentage of Peyer’s patches and mesenteric lymph nodes in the control group were significantly decrease（P〈0.05 or P〈0.01）.Compared with the sham operation group,the percentage of CD3＋T cells in control and Qihuang decoction group were significantly decrease（P〈0.01 or P〈0.05）.The percentage of CD3＋T cells in Qihuang decoction group was higher than control group（P〈0.01）.Compared with the sham operation group,the percentage of CD4＋T cells in control and Qihuang decoction group were significantly decrease（P〈0.01 or P〈0.05）.Qihuang decoction group compared with control group,has no significant difference（P〉0.05）.Compared with the sham operation group,the percentage of CD8＋T cells in control group were decrease（P〈0.05）.Qihuang decoction group compared with control group and sham operation group has no significant difference（P〉0.05）.Compared with the sham operation group,the percentage of IgA＋B cells in Peyer’s patches in control and Qihuang decoction group were significantly decrease（P〈0.01 or P〈0.05）.The percentage of IgA＋B cells in Qihuang decoction group was higher than the control group（P〈0.01）.[Conclusion]By promoting intestinal lymphocytes homing after surgery,Chinese medicine Qihuang decoction can protect the intestinal mucosal immune barrier,prevent and treat bacterial translocation.