摘要
[目的]通过检测治疗前、后胃黏膜上皮细胞凋亡、Foxo3a、FasL及胃镜变化,探讨舒胃汤防治胃溃疡的可能机制。[方法]选择符合诊断标准、纳入标准及排除标准的胃溃疡患者30例,予舒胃汤连续6周。应用电子胃镜检测治疗前、后溃疡变化;TUNEL染色检测治疗前后胃黏膜上皮细胞凋亡变化;以及Real-time PCR技术检测治疗前后Foxo3a、FasL、Caspase-8变化。[结果]从胃镜下溃疡修复分析,舒胃汤治疗肝胃郁热型胃溃疡总有效率为80%,治疗前胃黏膜上皮细胞凋亡率为14.36±2.71%,Foxo3a、FasL及Caspase-8mRNA表达分别为1.89±0.32、2.13±0.25、2.04±0.22;治疗后胃黏膜上皮细胞凋亡率为5.73±0.46%,Foxo3a、FasL及Caspase-8mRNA表达分别为1.02±0.11、1.13±0.19、1.21±0.09。[结论]舒胃汤可能通过下调Foxo3a、FasL及Caspase-8表达,延缓上皮细胞凋亡,促进黏膜修复,达到防治胃溃疡的效用。
[Objective]To access the prevention mechanism of Shuwei decoction on gastric ulcer by detecting the apoptosis of gastric mucosa epithelial cells and the expression of Foxo3 a,FasL and the changes under gastroscope before and after the treatment.[Methods]Thirty gastric ulcer patients who matched the diagnostic criteria,inclusion criteria and exclusion criteria were given Shuwei decoction for 6 weeks.The changes of ulcer were observed under the gastroscope before and after the treatment.The apoptosis of gastric mucosal epithelial cells was detected by TUNEL staining.The expression of Foxo3 a,FasL and Caspase-8were detected by Real-time PCR.[Results]Based on the analysis of ulcer repair under gastroscope,the total efficiency of Shuwei decoction was 80%.Before the treatment,the apoptosis rate of gastric mucosa epithelial cells was 14.36±2.71%.The expression of Foxo3 a,FasL and Caspase-8mRNA were 1.89±0.32.2.13±0.25.2.04±0.22,respectively.But after the treatment,the apoptosis rate of gastric mucosa epithelial cells was 5.73±0.46%.The expression of Foxo3 a,FasL and Caspase-8mRNA were 1.02±0.11,1.13±0.19,1.21±0.09 respectively.[Conclusion]Shuwei decoction can prevent the occurrence of gastric ulcer by reducing the expression of Foxo3 a,FasL and Caspase-8,delaying the epithelial cell apoptosis and promote mucosal repair.
出处
《中国中西医结合消化杂志》
CAS
2015年第7期458-461,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
广西卫生厅自筹经费项目(No:Z2014554)
