摘要
目的:观察灯盏生脉胶囊(DZSM)对大鼠脑缺血再灌注损伤模型的影响及机制。方法:采用大脑中动脉栓塞(MCAO)的方法建立大鼠脑缺血再灌注损伤模型。分为假手术组、MCAO组、DZSM组、甘珀酸(CBX)组、DZSM+CBX组。各组于再灌注后48 h评估大鼠神经功能缺损评分、梗死体积,测定Caspase-3表达量,MCAO组分别于再灌注后3、12、24、48 h观察缝隙连接蛋白43(Cx43)表达变化情况,再灌注后48 h比较各组Cx43表达量。结果:DZSM、CBX和DZSM+CBX组再灌注48 h神经功能缺损评分、脑梗死体积、Caspase-3表达量均低于MCAO组(P<0.05)。再灌注后各时间点Cx43表达量均升高(P<0.05),DZSM、CBX和DZSM+CBX组Cx43表达量较MCAO组低(P<0.05),CBX与DZSM+CBX组Cx43表达量较DZSM组低(P<0.05)。结论 :DZSM可减轻MCAO大鼠神经功能缺损评分,减少梗死体积,下调Caspase-3表达量,其机制可能与抑制Cx43表达有关。
Objective To investigate the neuroprotective effect of Dengzhan Shengmai capsule （DZSM） in rat cerebral ischemia-reperfusion injury and to explore the mechanism. Methods Rats were divided into Sham group, MCAO group, DZSM group, carbenoxolone （CBX） group and DZSM ＋ CBX group. Each group was assessed for neurological function, infarct volume and the expression of Caspase-3 48 h after reperfusion. Connexin 43 （Cx43） expression of MCAO group was detected 3, 12, 24, 48 h after reperfusion. Results There were lower neurological deficit scores, infarct volume and the expression of Caspase-3 in DZSM, CBX and DZSM ＋ CBX group 48 h after reperfusion when compared with those in MCAO group （P 〈 0.05） but Cx43 expression level in each group increased after repeffusion at each time point （P 〈 0.05）. Expression of Cx43 was lower in DZSM, CBX and DZSM ＋ CBX group than that in MCAO group （P 〈 0.05）. Lower expression of Cx43 was also seen in CBX and DZSM ＋ CBX group when compared with that in DZSM group （P 〈 0.05）. Conclusion DZSM capsule can improve neurological function, reduce infarct volume and inhibit the expression of Caspase-3. The mechanism may be related to its inhibition of Cx43 expression.
出处
《实用医学杂志》
CAS
北大核心
2015年第15期2430-2433,共4页
The Journal of Practical Medicine
基金
国家自然科学基金资助项目(编号:81403214)
