CD4^+CD29^+T细胞在肺癌患者的表达及其与肿瘤病理学类型及分期的相关性

Correlation between CD4^+CD29^+T cells and the pathological typing and staging for patients with primary pulmonary carcinoma

目的:探讨肺癌患者CD4+CD29+T细胞与病理类型及分期的相关性。方法:采用流式细胞术检测60例肺癌患者外周血CD4+CD29+T细胞、γ干扰素(IFN-γ)及肿瘤坏死因子α(TNF-α),探讨三者与病理类型及分期的相关性。结果:(1)肺癌患者CD4+CD29+T细胞及TNF-α百分含量均较对照组显著升高(P<0.05),而IFN-γ显著降低(P<0.05);(2)肺癌患者鳞癌、腺癌、大细胞癌及小细胞癌分别为23、17、11及9例;0、Ⅰ、Ⅱ及Ⅲ期患者分别为15、15、17及13例;(3)CD4+CD29+T细胞及TNF-α百分含量与病理类型及分期均呈显著正相关性,但IFN-γ与病理类型及分期呈显著负相关性(r绝对值均>0.65,均P<0.05)。结果:CD4+CD29+T细胞及TNF-α含量升高、IFN-γ降低是肺癌的一个重要免疫学特征,且与病理类型及分期密切相关。

Objective To explore the correlation between CD4^＋CD29^＋T cells and the pathological typing and staging for patients with primary pulmonary carcinoma. Methods Flow cytometry was used to detect peripheral blood CD4^＋CD29^＋T cells, gamma interferon （IFN-γ） and tumor neerdsis factor alpha （TNF-α） of 60 patients with lung cancer. The relationships between them and the pathological typing and staging were studied. Results （1） The CD4^＋CD29^＋T cellsand TNF-α percentages in thecancer patients were significantly higher than those of the control group （P 〈 0.05）, while IFN-γ/was significantly reduced （P 〈 0.05） ; （2） Amongthe cancer patients, there were 23 cases of squamous carcinoma, 17 cases of adenocarcinoma, 11 cases of large cell carcinoma and 9 cases of small cell carcinoma. The numbers of the cases at the stage of 0, Ⅰ , Ⅱ and Ⅲ were 15, 15, 17 and 13, respectively. （3） The percentages of CD4^＋CD29^＋T cells and TNF-α had significantly positive correlation with the pathological classification and staging, but IFN-γ-was negatively correlated with pathological typing and staging （r 〉 0.65, P 〈 0.05）. Conclusion Theincreased CD4＋CD29＋T cells and TNF-α, together with decreased IFN-γ/arehighly indicative of immunological characteristics of lung cancer, and closely related to the pathological typing and staging.