结核菌特异性IFN-γ Elispot检测在结核性脑膜炎患者诊断中的应用价值

The value of Mycobacterium tuberculosis specify Elispot in the diagnosis of tuberculous meningitis

目的评价结核菌特异性IFN-γElispot检测方法在结核性脑膜炎病人中的应用价值。方法采用结核菌特异性Elispot方法检测了96例健康对照、89例肺活动性肺结核病人和16例结核性脑膜炎病人的结核菌抗原特异性IFN-γ反应水平。结果 Elispot检测结核性脑膜炎病人IFN-γ水平显著高于健康对照组(P<0.001),但低于肺结核病人(P<0.05)。各组人群中的Elispot检测阳性率分别是肺结核病人89.89%、结核性脑膜炎病人62.5%、健康对照7.91%。结论采用Elispot检测结核菌特异性IFN-γ用于诊断结核性脑膜炎病人可行,具有较好的应用价值。

Objective To investigate the application value of interferon- gamma（ IFN- γ）measurement in the diagnosis of tuberculous meningitis by using in- house Elispot. Methods IFN- γ response in peripheral blood was determined in 96 healthy control（ HC）,89 patients with pulmonary tuberculosis（ PTB）and 16 patients with tuberculous meningitis（ TBM） by an in- house Elispot. Results By using Elispot,the level of IFN- γ in MTB was much higher than the healthy controls（ P〈 0. 001）,but lower than the PTB（ P 〈0.05）. The positive IFN- γ response in Elispot observed in HC,PTB and TBM were 89. 89%,62. 5% % and 7.91% respectively. Conclusions It is practical and reliable to differentiate the patients with TBM from healthy donors by measuring the Mtb antigen- specific IFN- γ response in the peripheral blood using our in- house Elispot assay.