醋酸杆菌生物催化丙烯醛合成丙烯酸

Biosynthesis of acrylic acid from acrolein by Acetobacter sp.

丙烯酸是一种重要的有机化工原料,主要用于合成树脂和丙烯酸酯,广泛应用于生产和生活的各方面.本文开展了利用微生物催化丙烯醛生产丙烯酸的研究.通过菌株筛选和ESI-MS验证,获得2株目的微生物,经鉴定比较发现Acetobacter sp.对丙烯醛的催化效率较高,在Acetobacter sp.细胞浓度15 g/L,初始底物浓度10 g/L,pH 6.8,30℃的条件下反应1 h,转化率可达88.0%,产物浓度达到11.32 g/L丙烯酸.同时,利用固定化细胞的方法提高反应对底物和产物的耐受性,经过2.5 h的反应,20 g/L的丙烯醛被催化成22.96 g/L丙烯酸,摩尔转化率为89.3%,固定化细胞重复利用6次后转化率仍可达到85.4%.利用Acetobacter sp.细胞催化生产丙烯酸,为丙烯酸的制备提供了一种新的可能.

As an important organic chemical material, acrylic acid is used to synthesize resin and acrylate, which is needed in various fields of life and production. To screen a strain capable of transforming acrolein to acrylic acid, two strains were isolated and identified by morphological analysis, biochemical properties and 16S rDNA gene sequences. Acetobacter sp. exhibited higher catalytic performance. At optimum conditions （cell concentration 15 g/L, acrolein concentration 10 g/L, pH 6.8, 30 oC）, 11.32 g/L acrylic acid was produced with molar transconversion of 88.0%. Immobilized cells method was applied to enhance the acrolein resistance of the strain. Sodium alginate of 2% and CaCl2 of 0.4% were used to entrap Acetobater cells. As a result, 20 g/L acrolein was catalyzed to 22.96 g/L acrylic acid in 2.5 h with molar conversion up to 89.3%. The productivity was up to 85.4% after the immobilized cells being reused for 6 times. This study provides a promising alternative of acrylic acid production.