郫县豆瓣中可培养细菌多样性分析及酶活性初筛

Isolation of Culturable Microorganisms in Pixian Bean Paste and Its Phylogenetic Diversity Analysis and Enzyme Activity Screening

为了将现代生物科学技术应用于传统食品生产的研究和改良,应用纯培养方法和基于16SrRNA基因序列的系统发育分析,对郫县豆瓣中的细菌进行了研究,对所得的219株纯培养细菌经去重后的35株典型菌株进行了16SrRNA基因测序和多样性分析。研究结果表明,郫县豆瓣中细菌多样性丰富,包含8个属,其中Bacillus是优势类群,占所有菌株比例达59.8%;另外还含有Myroides(11.4%),Providencia(5.72%),Alcaligenes(5.72%),Massilia(2.86%),Virgibacillus(2.86%),Morganella(2.86%),Geobacillus(2.86%),Fictibacillus(2.86%)。淀粉酶和蛋白酶筛选结果表明,豆瓣细菌具有良好的产酶活性,其中产蛋白酶的阳性菌株占70.1%,产淀粉酶阳性菌株占64.8%。该研究为豆瓣的大规模现代化生产提供了理论基础和生产材料。

In order to study and improve the application of modern biological science and technology to the traditional food production, the method of culture-dependent method and the phylogenetic analysis of 16S rRNA gene sequence are used. Microorganisms isolated from Pixian bean paste are studied. Thirty-five typical strains are sequenced and analyzed diversity duplicate removal from 219 cultured strains. The research results show that, Pixian bean paste has a rich microbial diversity, which including eight genera, including Bacillus which is the dominant group, accounting for 59.8%, and 11.4% for Myroides, 5.72% for Providencia, 5.72% for Alcaligenes, 2.86% for Massilia, 2.86% for Virgibacillus, 2. 86% for Morganella, 2. 86% for Geobacillus, 2. 86% for Fictibacillus. The results of screening of amylase and protease show that the microbial from bean paste with high enzyme activity , among which , the positive strains produce protease accounting for 70.1%%, the positive strains produce amylase accounting for 64. 8%. This study provides theoretical basis and production materials for large-scale modernized production.