大孔树脂纯化芫荽总黄酮及芫荽总黄酮抗氧化和抗菌活性研究

Study on the Antioxidant and Antimicrobial Activity and Purification with Macroporous Resin of Total Flavonoids from Coriandrum sativum L.

目的:研究芫荽总黄酮的大孔树脂纯化工艺及总黄酮抗氧化、抗菌活性。方法:通过筛选优化大孔树脂型号,优化大孔树脂提取芫荽总黄酮条件,利用DPPH芫荽总黄酮抗氧化活性,琼脂扩散法测试芫荽总黄酮抗菌活性。结果:AB-8树脂对芫荽总黄酮有较好的吸附和解吸效果,最优工艺条件为:芫荽总黄酮上样液的质量浓度为1.5 mg/mL,上样速率为2 mL/min,洗脱剂为70%乙醇,洗脱剂用量为4BV,洗脱速率为2mL/min,芫荽黄酮提取物清除DPPH·自由基的EC50为1.5mg/mL。对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌、绿脓杆菌、黑曲霉均有抗菌活性,但是对酿酒酵母无效。结论:AB-8型大孔树脂富集芫荽总黄酮效果最佳。

Objective： To investigate the purification technology of total flavonoids with macroporous resin and to study the antibacterial and antioxidant activity of total flavonoids from Coriandrum sativum L.. Method： Screen the optimal macroporous resin and optimize the purification technology of total flavonoids from Coriandrum sativum L. , then use the DPPH method to test the antioxidant activity and agar-diffusion method to test the antibacterial activity of total flavonoids from Coriandrum sativum L.. Results： AB-8 resin has excellent effect on the adsorption and desorption of total flavonoids from Coriandrum sativum L.. The sample concentration is 1. 5 mg/mL and the rate is 2 mL/min, the eluent is 70; ethanol of 4 BV and rate of 2 mL/min, which are the optimal purification process conditions. The total flavonoids from Coriandrum sativum L. also has excellent antioxidant activity （EC50 = 1. 5 mg/mL）, and the antibacterial activity against Bacillus subtilis, Staphylococcus aureus , Escherichia coli , Pesudomonas aeruginosa , Aspergillus niger, but not against Saccharomyces cerevisiae.