厚藤的组织培养及植株再生

Tissue Culture and Plantlet Regeneration of Ipomoeapes-caprae

以厚藤不同外植体为试材,MS为基本培养基进行组织培养,研究6-BA和NAA不同浓度组合对不同外植体愈伤组织诱导、不定芽分化及增殖的影响,以筛选出适合厚藤组织培养的最适外植体和培养基。结果表明：厚藤最佳的愈伤组织诱导培养基为MS＋6-BA 0.75mg/L＋NAA 0.25mg/L,最佳外植体为叶片;不定芽诱导培养基为MS＋6-BA 1.0mg/L＋NAA 0.1～0.5mg/L;在MS＋6-BA 1.0mg/L＋NAA 0.3mg/L培养基中增殖倍数最高。在1/2MS培养基上培养,生根率达87.5%。

Taking different explants of Ipomoea pes-caprae as material,using MS media with different combination of6-BA,NAA concentration,the effect of 6-BA,NAA on callus induction,bud initiation,shoot proliferation were studied to optimize the tissue culture of Ipomoea pes-caprae.The results showed that the initial medium for the callus induction was MS＋6-BA 0.75mg/L＋NAA 0.25mg/L,and the optimal explants were leaves.The best medium for adventitious bud differentiation was MS＋6-BA 1.0mg/L＋NAA 0.1-0.5mg/L.The best subculture media for shoot proliferation was MS＋6-BA 1.0mg/L＋NAA 0.3mg/L.The rooting rate was 87.5%in 1/2MS medium.