微波辅助云南鸡爪花根总黄酮提取工艺研究

Study on Extraction of Total Flavonoids From the Root of Jasminum duclouxii by Microwave-Assisted Route

以云南鸡爪花根为原料,乙醇溶液为提取剂,采用单因素试验和正交实验相结合方法,研究了料液比、乙醇浓度、微波加热时间及微波加热功率等工艺参数对总黄酮得率的影响。结果表明:鸡爪花根总黄酮的最佳提取工艺为料液比1∶20g/mL,乙醇浓度50%,提取时间60s,微波功率350W,总黄酮平均得率为2.21%。利用微波辅助法提取鸡爪花根总黄酮类物质,具有简单实用的特点,可大大缩短提取时间,达到省时、节能和高效的目的。

Taking Jasminum duclouxii as test materials,the technology of extraction of the total flavonoids from the root of Jasminum duclouxii was studied to provide a reference for it＇s further study and utilization,taking the root of Jasminum duclouxii as material,and alcohol as extractant,the effect of solid-1iquid ratio,ethanol concentration,extracting time and microwave power on the extraction of the total flavonoids from the root of Jasminum duclouxii was investigated using the methods of single factor test and orthogonal design test of L9（3^4）.The results showed that the highest extraction rate（2.21%）of the total flavonoids in the root of Jasminum duclouxii could be obtained under conditions of solid-1iquid ratio 1∶20g/mL,50%ethanol,extracting time 60 seconds,microwave power 350 W.The microwave-assisted extraction of flavonoids fromJasminum duclouxii was simple and practical,which was time saving,energy conserving and high efficient.