重楼皂苷Ⅶ联合二氧化硅纳米复合体对裸鼠卵巢癌的抑制及抗氧化作用

Establishment of nude mouse model with ovarian carcinomaand the effect of Paris Phyllin Ⅶ combined with silica nano complex on the inhibition and the antioxidant ability of ovarian carcinoma in nude mice

目的 建立裸鼠卵巢癌的研究模型,探讨重楼皂苷Ⅶ联合二氧化硅纳米复合体对裸鼠卵巢癌的抑制及抗氧化性的影响.方法 采用腋部皮下接种人卵巢癌耐药细胞SKOV3/DDP 200μl建立裸鼠卵巢癌模型.重楼皂苷Ⅶ联合二氧化硅纳米复合体灌胃处理裸鼠15 d,观察裸鼠体重变化和肿瘤抑制情况以及血清抗氧化能力的影响.结果 与阴性对照组相比,重楼皂苷二氧化硅纳米复合体处理组的肿瘤抑制率明显升高,肿瘤抑制率为58％,重楼皂苷处理组的肿瘤抑制率为36％,复合体处理组的肿瘤抑制率高于重楼皂苷处理组和二氧化硅纳米复合体单独作用的结果;血清SOD和T-AOC含量,重楼皂苷二氧化硅纳米复合体处理组显著高于对照组以及重楼皂苷和二氧化硅纳米复合体单独作用的结果;血清MDA,重楼皂苷二氧化硅纳米复合体处理组与阴性对照组相比显著降低.结论 重楼皂苷二氧化硅纳米复合体可能是通过增强抗氧化能力从而对裸鼠卵巢癌的抑制有一定的逆转作用.

Objective To establish the research model of ovarian carcinoma in nude mice,and to explore the effect of Paris Phyllin Ⅶ combined with silica nano complex on theinhibition and the antioxidant ability of ovarian carcinoma in nude mice.Method Nude mice modelswith ovarian carcinoma were established by axillary subcutaneous inoculation of human SKOV3/DDP resistant ovarian cancer cell 200 μl and were used in the experiment.Treating the nude mice with Paris Phyllin Ⅶ combined with silica nano complex by gavagefor 15 daystoobserve theweight change of the nude mice,tumor inhibition effect and changes of serum antioxidant capacity.Results Compared with the negative control group,tumorinhibition rate increased significantly in Paris Phyllin Ⅶ combined with silica nano complex treatment group,and was higher than that in both Paris Phyllin Ⅶ treatment only and silica nano composites treatment onlygroup.The serum superoxide dismutase （SOD） and total antioxidant capacity （T-AOC）level ofParis Phyllin Ⅶ combined with silica nano complex treatnent group was significantly higher than that of control group,Paris Phyllin Ⅶ treatment only and silica nano composites treatment onlygroup.The serum malonaldehyde（MDA） level of Paris Phyllin Ⅶ combined with silica nano complex treatment groupwas significantly lower than that of the negative control group.Conclusion Paris Phyllin Ⅶ combined with silica nano complex treatment can inhibit the ovarian carcinoma in nude mice,which may mediate by the enhancement ofantioxidant capability innude mice with ovarian cancer.