去甲肾上腺素转运体基因多态性与脑梗死患者抉择加工的关联性研究

Association between norepinephrine transporter gene polymorphism and decision-making processing in patients with cerebral infarction

目的探讨去甲肾上腺素转运体（NET）基因多态性与脑梗死患者抉择加工之问的关联性。方法采用聚合酶链反应技术（PCR）对145例脑梗死患者及188例健康对照者进行NET基因T182C和G1287A多态性检测。所有受试者均完成6种抉择情境问题的测试。结果脑梗死组和对照组NET—T182C基因型分布（χ2=4．437，P：0．049）及等位基因频率分布（χ2=4．363，P=0．037，OR=0．625，95％CI：0．436～0．895）和NETG1287A基因型分布（χ2=8．435，p=0．038）及等位基因频率分布（χ2=2．765，P=0．036，OR=1．520，95％CI：1．053～2．193）均差异有统计学意义。脑梗死组NETT182C三种基因型及携带T／C等位基因在6种抉择情境下，各方案选择概率比较差异均无统计学意义（均P〉0．05）。脑梗死组NET-G1287A及携带G／A等位基因在高风险与无风险损失情境（抉择情境4）下，其方案选择概率比较差异有统计学意义（P〈0．05，OR=1．657，95％CI：1．149～2．390），GG基因型患者选择高风险损失的概率明显低于其他两种基因型患者，G等位基因患者选择高风险损失方案的概率明显低于A等位基因的患者（P〈0．05），余5种抉择情境下各方案选择概率差异均无统计学意义（P〉0．05）。结论NET—G1287A多态性可能影响脑梗死患者抉择加工。GG基因型、G等位基因携带者，在高危险与无风险损失情境条件下，表现为更加损失风险规避。

Objective To investigate the association between norepinephrine transporter （NET） gene polymorphism and decision-making processing in patients with cerebral infarction. Methods A total of 145 patients with cerebral infarction and 188 normal controls were enrolled in our study. In all subjects, the polymerase chain reaction （PCR） for NET-T182C/G1287A polymorphism assay, gel electrophoresis, image analysis and enzymatic reaction, gene sequencing methods were used. The relationships of NET-T182C/G1287A genotypes and alleles with decision making processing were analyzed in patients with cerebral infarction. All participants completed six kinds of choice situational problems. Results There were significant differences in genotype and allele frequencies of T182C and G1287A polymorphism in NET between the patients with cerebral infarction and control group（for NET-T182C.. genotype, χ2= = 4. 437, P= 0. 049, allele frequency, χ2== 4. 363, P= 0. 037, OR= 0.625, 95%CI： 0.436-0.895;for NET-G1287A：genotype, χ2=8.435, P=0.038, allele frequency, χ2==2.765, P=0.036, ORE1.520, 95%Ch 1. 053 2. 193）. The cerebral infarction patients with three NET-T182C genotypes and T/C alleles all completed six choice scenarios, and the scheme selection probability had no significant difference （all P〉0.05）. In high risk and no-risk loss situation （scenario 4）, the scheme selection probability had significant difference in cerebral infarction patients with NET G1287A genotypes and G/A alleles （ P〈0.05 and 0.05, OR= 1. 657, 95% CI.. 1. 149 2.390）, and the patients with GG genotype tended to choose high-risk loss scheme, and the probability was obviously lower than that patients with other two genotypes, the patients with G allele tended to choose high-risk loss scheme, and the probability was obviously lower than that in patients with A allele （all P〈0.05）. In other five choice scenarios, the scheme selection probability had no significant difference between the patients （ all P 〉0.05 ） . Conclusions NET-G1287A polymorphism may be associated with decision-making processing in patients with cerebral infarction. In the high-risk and no-risk loss condition, patients with GG genotype and G allele have more loss risk aversion.