蚕沙总亚氨基糖类成分的富集纯化及提取物对α-葡萄糖苷酶的抑制活性

Enrichment,Purification and α-Glucosidase Inhibitory Activity of Total Iminosugars from Silkworm Faeces

为了开发利用蚕沙中的亚氨基糖类降血糖活性成分,采用阳离子交换树脂对蚕沙中的总亚氨基糖类成分进行富集纯化。以1-脱氧野尻霉素(DNJ)的含量作为考察指标,在筛选阳离子交换树脂种类的基础上进一步优化富集纯化工艺条件,并对总亚氨基糖类提取物进行α-葡萄糖苷酶活性抑制试验。001×7强酸型(H+)阳离子交换树脂对蚕沙总亚氨基糖类成分的富集效果最好,最佳富集纯化工艺为:按1 g阳离子交换树脂、1 g蚕沙提取液的比例上样,依次以p H 1的HCl溶液、水、0.5mol/L氨水洗脱,收集氨水洗脱液;将得到的氨水洗脱部分再以中性氧化铝纯化后,以50%乙醇洗脱;乙醇洗脱液60℃减压浓缩即得纯化的蚕沙总亚氨基糖类提取物。采用上述工艺条件从蚕沙中富集纯化的总亚氨基糖类提取物对α-葡萄糖苷酶的IC50为(0.056±0.002)mg/m L,具有良好的降血糖活性。

To develop and utilize iminosugar-hypoglycemic components in silkworm faeces,total iminosugars in silkworm faeces was enriched and purified with cation exchange resin. Taking the content of 1-deoxynojirimycin（ DNJ） as the evaluation index,the enrichment and purification technology was optimized on the basis of cation-exchange resin screening,and α-glucosidase inhibitory activity of total iminosugar extract was determined. The results showed that001 × 7 strong acid（ H^＋） cation-exchange resin had the best enrichment effect to total iminosugar in silkworm faeces.The optimal procedure for enrichment and purification of total iminosugar in silkworm faeces was as follows： the proportion of sampling was cation-exchange resin 1 g and silkworm faece extract solution 1 g; eluted with hydrochloric acid solution（ pH 1）,water and 0. 5 mol / L ammonia,respectively,and collected ammonia eluate; the obtained ammonia eluate was purified with neutral alumina,and then eluted with 50% ethanol; after ethanol eluate was concentrated at low pressure under 60 ℃,the purified total iminosugar extract in silkworm faeces was obtained. IC50 of total iminosugars extract produced by the above process toα-glucosidase was（ 0. 056 ± 0. 002） mg / m L,showing good hypoglycemic effect with α-glucosidase inhibitory activity.