载脂蛋白E基因多态性与散发性阿尔茨海默病的关系

Association between the apolipoprotein E gene polymorphism and the genetic sensitivity of sporadic Alzheimer's diseases.

目的 探讨载脂蛋白E(apoE)基因多态性与散发性阿尔茨海默病 (SAD)遗传易感性的关系。 方法 应用限制性片段长度多态分析 聚合酶链反应 (RFLP PCR)方法 ,测定 5 8例SAD和 6 0例同龄对照者apoE基因型和等位基因 ,并与 5 9例血管性痴呆 (VD)患者进行对比研究。 结果 SAD组ε4等位基因频率 (19 82 % )和 3/ 4型频率 (2 5 86 % )均明显高于对照组 (5 0 0 %和 6 6 7% ;χ2 =13 87,P <0 0 1)和VD组 (6 70 %和 10 17% ;χ2 =11 5 ,P <0 0 5 ) ,VD组ε4频率 (6 70 % )与对照组比较 ,差异无显著性 (χ2 =11 6 1,P >0 0 5 )。 结论 apoEε4等位基因是SAD的遗传易感基因。

Objective To study the relationship between apoE alleles and the occurrence of sporadic Alzheimer's disease (SAD) and to compare the alleles relating to the SAD and the vascular dementia (VD). Methods The polymorphism of apoE gene was detected using polymerase chain reaction restriction fragment length polymorphism(PCR RFLP) technique for 58 patients with SAD, 59 patients with VD and 60 normal controls. Results The frequencies of the ε4 and 3/4 genotype in SAD group(19 82% and 25 86%)were significantly higher than those in the controls(5 00% and 6 67%, χ 2=13 87, P <0 01), and those in VD(6 70% and 10 17%, χ 2=11 5, P <0 05). No significant difference was found when frequence of ε4 of the VD group (6 70%) were compared with that of the control group( χ 2=11 61, P >0 05). Conclusions The apoE ε4 allele is a genetic risk factor for the occurrence of SAD.