HPLC-DAD法同时测定清乳通贴膜剂中绿原酸和黄芩苷的含量

Determination of Chlorogenic acid and Baicalin in Qingrutong transdermal patch by HPLC-DAD

目的:建立清乳通贴膜剂中绿原酸和黄芩苷含量测定的方法。方法:采用反相高效液相色谱法,色谱柱:Agilent ZORBOX SB-C18色谱柱(5μm,4.6 mm×250 mm),流动相:乙腈(A)-0.2%磷酸水溶液(B),线性梯度洗脱[0-30min,A-B(20∶80)→A-B(60∶40)],流速:1.00 m L/min,柱温:40.0℃。检测器:二极管阵列检测器,检测波长:绿原酸327.0 nm、黄芩苷280.0 nm。结果:绿原酸和黄芩苷线性范围分别为0.14-1.39μg(r=0.999 6)、0.29-2.87μg(r=0.999 9);平均加样回收率(n=6)分别为98.71%(RSD=0.39%)、99.95%(RSD=0.49%)。结论:本方法简捷快速,结果准确可靠,适用于清乳通贴膜剂的质量控制。

Objective： To develop a method for the content determination of Chlorogenic acid and Baicalin in Qingrutong transdermal patch. Methods： The analytical column was a Agilent ZORBOX SB-C18 （5μm,4.6 mm×250 mm）. The mobile phase consisted of acetonitrite （A）-0.2% orthophosphorie acid （B） with the gradient elution [0→30min,A-B（20∶80）→A-B（60∶40）] .The flow rate was 1.0 mL·min^-1 and the column temperature was 40 ℃ .The detector was DAD. The detection wavelength was 327.0 mn for Chlorogenie acid and 280.0 nm for Baicalin. Results： The calibration curve of Chlorogenie acid and Baicalin was in good linearity over the range of 0.14-1.39 μg （r=0.999 6） and 0.29-2.87 μg （r=0.999 9）, and the average recovery （n=6） was 98.71% （RSD=0.39%） and 99.95% （RSD=0.49%）, respectively. Conclusion： The method is simple, rapid and reliable and can be used for the drug control.