摘要
建立了大黄末中大黄蒽醌含量测定的高效液相色谱方法。采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),甲醇-0.1%磷酸溶液(85∶15)为流动相,流速1.0 m L/min,荧光检测器检测,激发波长为442 nm,发射波长为514 nm。大黄酸、大黄素、大黄酚的浓度分别在0.2108-10.540 0μg/m L(r=0.999 9)、0.200 0-10.000 0μg/m L(r=0.999 6)、0.209 2-10.460 0μg/m L(r=0.999 5)范围内线性关系良好;平均回收率(n=3)分别为103.5%(RSD为3.0%)、102.3%(RSD为2.6%)、98.76%(RSD为2.9%)。结果提示,本方法准确灵敏,可用于大黄末的质量控制。
The HPLC method was established for the determination of Rhubarb anthraquinone in Rhubarb powder. A chromatographic column of Agilent Eclipse XDB-C18 (250 mm×4.6 mm,5μm) was used with the mobile phase of methanol - 0.1% phosphoric acid solution (85:15) at the flow rate of 1.0 mL/min. The compound was determined at λex 442 nm and λex 514 nm by fluorescence detector. The calibration curves were linear in ranges of 0.210 8-10.540 0 μg/mL (r= 0.999 9) for rhein, 0.200 0-10.000 0 μg/mL(r=0.999 6) for emodin, 0.209 2-10.460 0 μg/mL (r=0.999 5) for chrysophanol. The average recoveries (n=3) of rhein, emodin and chrysophanol were 103.5% (RSD= 3.0%), 102.3% (RSD=2.6%) and 98.76% (RSD= 2.9%), respectively. The method is accurate and sensitive for the quality control of Rhubarb powder.
出处
《中兽医医药杂志》
2015年第4期47-49,共3页
Journal of Traditional Chinese Veterinary Medicine
基金
浙江省农业厅中青年科技创新计划项目