卵巢切除与血清肿瘤坏死因子-α、转化生长因子-β_1浓度的关系

Experimental study of the relationship between ovary excision and the levels of TNF-αand TGF-β_1 in serum

目的 探讨卵巢摘除与血清肿瘤坏死因子 α(TNF α)、转化生长因子 β1(TGF β1)浓度的关系。 方法 30只SD大鼠随机分为模型Ⅰ (OVXⅠ )组、模型Ⅱ (OVXⅡ )组和假手术组 ,OVXⅠ、OVXⅡ组大鼠卵巢切除 ,术后 12和 2 4周运用酶联免疫法检测大鼠血清中TNF α、TGF β1浓度的变化。 结果 大鼠去卵巢后当雌二醇 (E2 )水平下降时 ,血清TNF α浓度〔(5 3 88± 16 35 )ng/L〕升高 ,而TGF β1含量〔(6 78± 0 75 ) μg/L〕下降 ,与假手术组比差异有显著性 (P <0 0 5或P <0 0 1) ;相关性分析显示 ,E2 与TNF α呈负相关 (r=- 0 384 ,P =0 0 5 ) ,而与TGF β1正相关 (r=0 75 0 ,P =0 0 0 1) ,两细胞因子之间亦呈负相关 (r=- 0 4 6 5 ,P =0 0 5 )。 结论 E2 对TNF α有抑制作用 ,但却能促进TGF β1的分泌 ,TNF α含量的增加和TGF β1水平的降低 ,均能刺激骨的吸收活动 ,导致骨质疏松症的发生。

Objective To investigate the relationship between ovary excision and serum levels of TNF α, TGF β 1. Methods Serum levels of TNF α and TGF β 1 in ovariectomized rats were measured by ELISA. Results The serum levels of TNF α were increased〔(53 88±16 35)ng/L〕, but the TGF β 1 were decreased 〔(6 78±0 75)μg/L〕 in ovariectomized rats when E 2 decreased compared with those of the Sham group( P <0 05, P <0 01) . At the same time, the serum levels of E 2 were negatively correlated with the TNF α, but positive correlated with the TGF β 1. Conclusions E 2 can inhibite the secretion of TNF α, but promote the secretion of TGF β 1, then stimulate the bone resorption and bring about the osteoporosis.