欧洲李‘红艳1号’的组织培养与快速繁殖

Tissue Culture and Rapid Proliferation of Prunus domestica Variety ‘Hongyan 1'

为了建立欧洲李新品种‘红艳1号’的组织培养快繁技术体系,研究了基本培养基及植物生长物质对腋芽启动、试管苗增殖及生根的影响。结果表明:以半木质化的嫩枝茎段为外植体进行腋芽的启动培养,基本培养基QL比MS有效。增殖培养时MS最佳,其次为QL,WPM和1/2MS较差。壮苗培养时WPM最优,其次为MS,1/2MS和QL较差。生根培养时1/4MS比1/2QL有效,最佳生根培养基为1/4MS+1 mg·L-1 IBA+20 g·L-1蔗糖,生根率为84%。

In order to establish the technology system of tissue culture and rapid proliferation of Prunus domestica variety ＇Hongyan 1＇, the effects of basal medium and plant growth substances on initial culture of axillary buds, proliferation and rooting of in vitro shoots were examined. Results showed that basal medium QL was more effective than MS in inducing the initial growth of axillary buds when half lignification stem section was selected as explants. For proliferation, basal medium MS was better, followed by QL, and WPM and I/2MS were poorer. For vigorous shoots culture, basal medium WPM was the optimal, followed by MS, and 1/2MS and QL were poorer. For in vitro rooting, basal medium 1/4MS was more effective than 1/2QL. The optimal rooting medium was 1/4MS＋1 mg·L^-1 IBA＋20 g·L^-1 sucrose, the rooting percentage reached 84%.