大黄素搽剂对兔耳增生性瘢痕的影响

Influence of Emodin Gel on the Fibroblasts of Hypertrophic Scars in Rabbit Ear Model

为研究大黄素搽剂(大黄素)对兔耳增生性瘢痕的影响,选择健康兔18只,于兔耳增生性瘢痕造模成功后,随机分为大黄素组(9只)和对照组(9只),大黄素组采用大黄素局部用药,对照组不进行治疗。4周后,测两组瘢痕直径、硬度、转化生长因子-β(TGF-β)、白细胞介素-1(IL-1)含量,并在电镜下观察瘢痕组织成纤维细胞超微结构变化。发现用药后大黄素组与对照组相比,兔耳增生性瘢痕直径差异无统计学意义(P>0.05),硬度、TGF-β及IL-1表达水平明显降低(P<0.05,P<0.01,P<0.05),电镜观察,大黄素组瘢痕成纤维细胞及细胞器减少,胶原纤维溶解。研究指出大黄素在瘢痕形成早期,可降低兔耳增生性瘢痕硬度,具有抑制局部成纤维细胞增殖的能力,有望成为防治增生性瘢痕的方法之一。

This research was aimed to study the effect of Emodin gel on the hypertrophic scars of rabbit ears. A total of 18 rabbits were randomly divided into Emodin group （9 rabbits） and control group （9 rabbits） after the successful animal model for hypertrophic scars had been made. The rabbits in the Emodin group were treated with Emodin Gel, while no special treatment was given to those in the control group. The other living conditions were all kept the same in the two groups. The diameter,hardness, and expression of transforming growth factor-β （TGF-β） and interleukin- 1 （IL-1） of hypertrophic scars were measured after 4 weeks. Transmission electron microscopy was applied to observe the ultra-structure of the fibroblasts of hypertrophic scars. But there was no difference between the two groups in the diameter of hypertrophic scars （P〉0.05）. The hardness, expression of TGF-β and IL-1 in hypertrophic scars in the Emodin group decreased, compared to the control group （P〈0.05, P〈0.01, P〈0.05）. Transmission electron microscopy showed that the fibroblast and organelle lessened in the cytoplasm and the collagen fibers dissolved obviously. The study showed that Emodin gel decreased the hardness of hypertrophic scars in the rabbit ears, and inhibited the proliferation of fibroblasts in local area. Therefore, Emodin gel treatment would be one of the methods to prevent and treat hypertrophic scars.