ABO血型亚型检测与血清学

Analysis of ABO blood group subtype identification and serology

目的通过对ABO血型亚型的检测和血清学分析,探讨ABO亚型的输血安全。方法选取本院输血科43例患者的正反型不符血液标本,检测红细胞ABH抗原、ABO血型系统抗体;并用ABO正反定型、唾液血型物质测定、吸收释放试验等进行血清学检查。结果 43例标本共检出ABO血型亚型13种,其中A亚型8例(18.60%),B亚型32例(78.05%),类孟买型1例,cis AB型2例(4.65%)。结论对于ABO血型亚型的鉴定应采用多种血清学方法进行检测,为临床输血提供安全保障。

Objective To investigate the transfusion safety of ABO blood group subtype through ABO blood group subtype identification and serology analysis. Methods 43 patients whose positive and negative type did not match the blood specimen were chosen.Their ABH antigen of red blood cells and antibody of ABO blood group system were detected.Their serology was checked with application of ABO positive and negative stereotypes,determination of blood group substances in saliva,absorption and release test and so on. Results In these 43 cases,13 cases were diagnosed with ABO blood group subtype,among which 8 were A subtype（18.60%）, 32 were B subtype（78.05%）,l was Para-Bombay type and 2 were cisAB type（4.65%）. Conclusion Blood group identification of ABO blood group subtype should be checked by a variety of serological methods,which guarantees the safety of clinical blood transfusion.