摘要
为开发环境相容性好、贮藏稳定性高的生物农药新剂型,采用喷雾干燥法制备了枯草芽孢杆菌微囊剂。通过单因素试验确定该微囊剂壁材麦芽糊精与芯材枯草芽孢杆菌发酵液(以下简称发酵液)的最佳配比;通过正交试验优化了喷雾干燥条件;通过田间试验验证了该微囊剂对番茄立枯病的防治效果。结果表明:制备枯草芽孢杆菌微囊剂时,壁材与芯材的最佳配比为m(麦芽糊精)∶V(发酵液)=1∶1;喷雾干燥的最优条件为进风温度125℃,进样流量750 m L/h,喷雾压力0.20 M Pa;所制备微囊剂常温贮存360 d后,菌体存活率仍高达91.36%,显著高于对照枯草芽孢杆菌可湿性粉剂。田间药效试验表明,该枯草芽孢杆菌微囊剂用量为300 g/hm2(芽孢浓度为1.5×107cfu/m2)时,对番茄立枯病的防效最高,为72.76%。
In order to develop compatible and stable newbiological pesticide,microencapsulation of Bacillus subtilis was prepared by spray-drying method. The optimum spray-drying conditions were determined by orthogonal test,and the biocontrol efficacy against Rhizoctonia solani in tomato under field conditions was studied. The results suggested that the best proportion of maltodextrin and fermentation broth was 1∶ 1（ m ∶ V）. The optimum condition of spray-drying was： inlet air temperature125 ℃, flowrate 750 m L / h, and spray pressure 0. 20 MPa. The survival rate of B. subtilis microcapsules was 91. 36% after storage for 360 days,which was significantly higher than that of B.subtilis wettable powders. The result of field trial showed that the biocontrol efficacy of B. subtilis microcapsules against tomato R. solani could reach 72. 76% when the application dosage was300 g / hm2.
出处
《农药学学报》
CAS
CSCD
北大核心
2015年第4期462-468,共7页
Chinese Journal of Pesticide Science
基金
上海市重点攻关项目(12391901800)
国家星火计划项目(2013GA680001)
