固相萃取-高效液相色谱法同时测定伤疡愈软膏中两种成分含量

Determination of Two Components in Shangyangyu Ointment by Solid Phase Extraction Coupled with HPLC Method

目的:建立固相萃取-高效液相色谱法,同时测定伤疡愈软膏中紫草的主要成分β,β'-二甲基丙烯酰阿卡宁和丹参中丹酚酸B的含量。方法:采用固相萃取法和高效液相色谱法联用,选用C18(250 mm×4.6 mm,5μm)色谱柱;以乙腈-水-甲醇-甲酸(700∶300∶30∶15)为流动相;流速:1.0 ml·min^-1;β,β'-二甲基丙烯酰阿卡宁检测波长:275 nm,丹酚酸B检测波长为286 nm的相同系统下同时测定伤疡愈软膏中紫草的主要成分β,β'-二甲基丙烯酰阿卡宁和丹参中丹酚酸B的含量。结果:β,β'-二甲基丙烯酰阿卡宁浓度在23.780~118.900μg·ml-1范围内线性关系良好(r=0.999 5),回收率为99.5%,RSD=1.07%;丹酚酸B在19.840~99.200μg·ml-1范围内线性关系良好r=0.999 3,丹酚酸B的平均回收率为98.2%,RSD=2.1%(n=9)。结论:该方法操作简单,快速,分离度好,建立的定量方法可用于伤疡愈软膏的质量控制。

To develop a determination method forβ,β＇-dimethylacrylalkannin and salvianolic acid B in Shangyangyu ointment by solid phase extraction coupled with HPLC. Methods：Solid phase extraction coupled with HPLC was used with a C18 （250 mmx 4. 6 mm, 5 μm） column, the mobile phase was acetonitrile-water-methanol-formic acid （700∶300∶30∶15） with the flow rate of 1. 0 ml·min-1. For β, β＇-dimethylacrylalkannin, the detection wavelength was 275nm, and for salvianolic acid B, the detection wavelength was 286nm. Under the above conditions, the contents of main componentsβ,β＇-dimethylacrylalkannin and salvianolic acid B in Shangyangyu ointment were determined. Results：The linear relationship was promising when the concentration ofβ,β＇-dimethyl-acrylshikonin was within the range of 23. 780-118. 900μg·ml-1（r=0. 999 5） with the recovery of 99. 5% （RSD=1. 07%） and that of salvianolic acid B was within the range of 19.840-99.200 μg·ml-1（r=0.999 3） with the recovery of 98.2% （RSD=2.1%）. Conclusion：The method is simple, accurate and fast with good separation, which can be used in the quality control of Shangyangyu ointment.