《中国药典》中桑叶含量测定项下方法的改进

Improvement of Assay Method for Mori Folium in Chinese Pharmacopoeia

目的:对《中国药典》中桑叶含量测定项下方法进行改进。方法:采用HPLC法,Agilent Zorbax SB-C18色谱柱(250mm×4.6 mm,5μm);流动相为乙腈-0.2%磷酸水溶液(梯度洗脱),流速为1.0 ml·min^-1;检测波长为354 nm;柱温为30℃。结果:《中国药典》2010年版桑叶含量测定项下测得的芦丁含量实为芦丁和异槲皮苷的含量之和,优化后方法可分离该两种化合物,且芦丁和异槲皮苷分别在2.76~27.60μg·ml-1(r=0.999 9)和4.74~47.39μg·ml-1(r=0.999 8)范围内与峰面积呈良好的线性关系;平均加样回收率分别为100.31%(RSD=0.83%)和100.32%(RSD=1.04%)(n=6)。结论:优化后方法简便、稳定、重复性好,可用于桑叶的质量控制。

To optimize the extract method and chromatographic conditions for the determination of rutin in Mori Folium in Chinese pharmacopoeia. Methods：The HPLC analysis was performed on a ZORBAX SB-C18 （250 mmx 4. 6 mm, 5 μm） column. The mobile phase was acetonitrile-0. 2% phosphorice acid solution with gradient elution and the flow rate was 1. 0 ml·min-1 . The de-tection wavelength was 354 nm, and the column temperature was 30 ℃. Results： The content of rutin determined by the method in Chinese pharmacopoeia was actually the total contents of rutin and isoquercitrin, while the optimized method could separate the two components effectively, and the good linearity of rutin and isoquercitrin was within the range of 2.76-27.60 μg·ml-1（r=0.999 9） and 4. 74-47. 39 μg·ml-1（r=0. 999 8）, respectively, and the average recovery was 100. 31% （RSD=0. 83%） and 100. 32%, re-spectively （RSD=1. 04%, n=6）. Conclusion：The optimized method is simple, stable and reproducible,which can be used in the quality control of Mori Folium.