一测多评法测定肉苁蓉中2种有效成分的含量

Simultaneous Determination of Two Effective Components in Cistanche by Quantitative Analysis of Multicomponents by Single-marker

目的:建立同时测定肉苁蓉中松果菊苷、毛蕊花糖苷的高效液相色谱一测多评法。方法:以松果菊苷和毛蕊花糖苷为考察指标,建立两者间的相对校正因子,并用校正因子计算另一个成分的含量,将一测多评法的计算值与外标法实测值进行比较。结果:建立的相对校正因子重现性良好,采用校正因子计算的含量值和外标法实测值之间没有显著性差异。结论:同时测定肉苁蓉中2种成分的一测多评法方法可靠,结果准确,可用于控制肉苁蓉药材及其饮片的质量。

To establish a determination method for two effective components echinacoside and acteoside in cistanche by quantitative analysis of multi-components by single marker （ QAMS） . Methods：With echinacea glycosides and mullein indican as the examining indices, the relative correction factor （ RCF） between echinacea glycosides and mullein indican was established, and then used to calculate the content of the other components. Meanwhile, the calculated value was compared with that of the external standard method. Results：The RCFs had good reproducibility without significant differences between the quantitative results of QAMS method and those of the external standard method. Conclusion：The described method is reliable and accurate, and can be used to control the quality of echinacoside and acteoside in cistanche.