纯钛钛片表面不同生物大分子涂层的比较研究

Comparative study of different biomolecule coatings on the surface of pure titanium disc

目的探讨如何在纯钛钛片表面构建不同生物大分子涂层,以期找到合适的种植体表面涂层。方法纯钛钛片表面经打磨抛光及氧化性混酸溶液处理(酸蚀组),设为对照,接着通过层层自组装技术将Ⅰ型胶原(COL)、透明质酸(HA)、壳聚糖(CHI)和多聚谷氨酸(PGA)引入到钛片表面(涂层组)。采用扫描电镜(SEM)、X射线光电子能谱(XPS)、石英晶体微量天平(QCM)和接触角试验对钛片的表面进行表征。在各种钛片表面培养成骨前体细胞,检测其早期黏附、增殖及分化的能力。结果SEM结果显示,各组涂层的表面非常相似、平整。XPS分析发现各组钛片表面元素成分氮的含量随着组装层数的增加而不断升高。接触角实验显示在纯钛表面组装生物大分子并没有损害基底面良好的亲水性。涂层组钛片较酸蚀组明显促进了成骨细胞的早期黏附、增殖和分化。胶原组[Ti-(COL/HA)5-COL、Ti-(COL/PGA)5-COL]较壳聚糖组[Ti-(CHI/HA)5-CHI、Ti-(CHI/PGA)5-CHI]具有更高的生物活性。而Ti-(COL/HA)5-COL与Ti-(COL/PGA)5-COL相比更能促进成骨细胞的黏附、增殖和分化。结论生物大分子COL、HA、CHI和PGA能够通过LBL技术被成功地引入到纯钛钛片表面。Ti-(COL/HA)5-COL具有较高的生物活性,可能有利于种植体的骨整合。

Objective To construct different biomolecule coatings on the surface of pure titanium discs and to find out a proper implant coating. Methods Pure titanium disc was first ground,polished and acid-etched with a mixture of H2SO4 /H2O2（ acid-etched group,as the control group）. Collagen typeⅠ（ COL）,chitosan（ CHI）,hyaluronic acid（ HA） and polyglutamic acid（ PGA） were introduced onto this acid-etched surface using layer by layer technique（ coating group）. Scanning electron microscope（ SEM）,X-ray photoelectron spectroscopy（ XPS）,quartz crystal microbalance（ QCM） and contact angle measurement were used for surface characterization. After pre-osteoblasts were seeded on the discs,cell attachment,proliferation and differentiation were evaluated. Results SEM analysis demonstrated that the surfaces of different coatings were very smooth and similar. XPS analysis showed that the nitrogen content of different surface was dramatically increased with deposition number. Contact angle measurement showed that the construction of different biomolecules on pure titanium discs did not damage the excellent wettability of the basal surface. Cell attachment,proliferation and differentiation were much stimulated in the coating groups when compared with the acid-etched group.On the other hand,COL-based coatings [Ti-（ COL /HA）5-COL and Ti-（ COL /PGA）5-COL]showed much higher bioactivity compared with CHI-based coatings [Ti-（ CHI /HA）5-CHI and Ti-（ CHI /PGA）5-CHI],and Ti-（ COL /HA）5-COL coating promoted cell attachment,proliferation and differentiation much more than those of Ti-（ COL /PGA）5-COL coating.Conclusion Collagen typeⅠ,chitosan,hyaluronic acid and polyglutamic acid are successfully constructed on the titanium surface,and Ti-（ COL /HA）5-COL coating shows high bioactivity and may promote implant osseointegration.