心肺复苏术后患者血清神经元特异性烯醇化酶、肿瘤坏死因子-α和白介素-6的变化及意义

The Changes and Clinical Significance of Serum TNF- α、IL-6 and NSE in Patients after Cardiopulmonary Resuscitation

目的:探讨心肺复苏术后患者血清中神经元特异性烯醇化酶(NSE)、肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)的水平变化及其临床意义。方法:采用酶联免疫吸附法测定26例心肺复苏术后患者各时间点血清TNF-α、IL-6、NSE的水平变化。结果:心跳骤停患者在自主循环恢复后血清TNF-α、IL-6和NSE的水平逐渐升高,TNF-α在自主循环恢复后24 h达到高峰,而IL-6和NSE的水平在自主循环恢复后48 h才达到高峰;且血清IL-6与TNF-α水平呈显著正相关(P<0.01);血清NSE与TNF-α、IL-6的水平均呈显著正相关(P<0.01)。结论 :TNF-α、IL-6介导的炎症反应过程参与了心跳骤停患者自主循环恢复后机体的病理损伤过程,且脑损伤严重程度与血清TNF-α、IL-6的水平密切相关。

Objective ：To explore the change and clinical significance of serum tumor necrosis factors- α（TNF-α）、interleukin-6（IL-6） and neuron-specific enolase（NSE）in patients after cardiopulmonary resuscitation（CPR）.Methods： The levels of serum TNF-α、IL-6 and NSE at each time point in 26 patients after CPR were measured by ELISA. Results ：The levels of serum TNF-α 、IL-6 and NSE gradually increased in patients after CPR,the peak value of serum TNF-α appeared at 24 hours after CPR,the peak value of serum IL-6 and NSE appeared at 48 hours after CPR. The serum IL-6 levels were positively correlated with serum TNF-αlevels（P〈0.01）,and the serum NSE levels were positively correlated with serum TNF-αand IL-6 levels（P〈0.01）. Conclusion ：TNF-α、IL-6 may be involved in the pathological damage process of patients after CPR,and brain injury severity may be closely associated with the levels of serum TNF-α、IL-6 in patients after CPR.