过氧化氢酶对吸烟相关喉鳞状细胞癌发病的影响

The effect of catalase on smoking related laryngeal squamous cell carcinoma

目的:探讨氧化应激在吸烟相关喉鳞状细胞癌发生发展中的影响,以及过氧化氢酶(CAT)对吸烟相关喉鳞状细胞癌的临床诊断意义。方法:收集吸烟人群119例,其中吸烟相关喉鳞状细胞癌组68例、非喉鳞状细胞癌人群(对照组)51例,比较2组血浆和癌组织的CAT、丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、一氧化氮(NO)水平,分析氧化应激指标与吸烟相关喉鳞状细胞癌及其严重程度的关系。结果:1与对照组相比,喉鳞状细胞癌组的吸烟密度、吸烟支数、CAT、MDA、GSH显著升高(均P<0.01);而SOD、NO明显下降(均P<0.01)。2随着喉鳞状细胞癌组织分化程度越低,血清CAT越高(P<0.01),癌组织匀浆的CAT、MDA、GSH也越高(均P<0.01),而血清NO则越低(P<0.01),癌组织匀浆的SOD、NO越低(均P<0.01);喉鳞状细胞癌患者临床分期越高,血清和癌组织的CAT、癌组织的GSH越高(均P<0.01),而癌组织NO越低(P<0.01)。3血清CAT、MDA为吸烟相关喉鳞状细胞癌发病的独立危险因素(OR=1.060,2.475;P<0.01,P<0.05)。结论:氧化应激是吸烟人群发生喉鳞状细胞癌的主要影响因素,CAT可作为吸烟相关喉鳞状细胞癌的临床诊断指标。

Objective：Investigate the effect of oxidative stress on the occurrence and development of laryngeal squamous cell carcinoma associated with smoking,and the clinical diagnostic value of catalase on smoking related laryngeal squamous cell carcinoma.Method：Collecting 119smokers（including the smoking related laryngeal cancer group 68 cases,the control group 51cases）,the indexes of catalase（CAT）,malondialdehyde（MDA）,superoxide dismutase（SOD）and glutathione（GSH）,nitric oxide（NO）in blood plasma and cancerous tissue in two groups were compared.The association between these oxidative stress indicators and the occurrence and severity of smoking related laryngeal squamous cell carcinoma was analysised by SPSS 17.0.Result：1 Compared with control group,the smoke frequency and amount,CAT,MDA,GSH increased significantly in the smoking related laryngeal cancer group（P=0.000;0.000;0.000;0.000;0.000）;whereas SOD,NO decreased（P=0.000;0.000）.2The lower the differentiation degree,the higher the serum CAT（P=0.000）and the higher CAT,MDA,GSH of larynx tissue（P=0.000;0.000;0.000）,but the lower the serum NO（P=0.000）and the lower SOD,NO of larynx tissue（P=0.000;0.000）;The higher the clinical stage,the higher CAT of serum and larynx tissue and the higher GSH of larynx tissue（P=0.000;0.001）,the lower NO of larynx tissue（P=0.009）.3The serum CAT,MDA were independent risk factors of smoking related laryngeal squamous cell carcinoma（OR=1.060,2.475;P〈0.01,P〈0.05）.Conclusion：Oxidative stress is the key factor of the occurrence of smoking related laryngeal squamous cell carcinoma,and the CAT can be used as the indicator of clinical diagnosis of smoking related laryngeal squamous cell carcinoma.