Hedgehog通路抑制基因在非小细胞肺癌中甲基化的研究

Hedgehog pathway suppressor gene methylation in non - small cell lung cancer

目的探讨Hedgehog通路（HH）抑制基因的PTCH蛋白在非小细胞肺癌（NSCLC）组织的甲基化表达以及其临床意义。方法应用甲基化特异性聚合酶链反应（MS—PCR）进行检测NSCLC中信号传导通路相关抑癌基因的甲基化状态。结果HH通路抑制基因在NSCLC中特异性不一，GAS基因的特异性为82．61％；HHIP的特异性为69．57％。PTCH的特异性为100．00％，差异均有统计学意义（P〈0．01）。结论HH通路抑制基因在肺腺癌和鳞癌中可能有不同的作用机制，住腺癌中可能通过旁分泌调控PTCH活性，而在鳞癌中则可能是通过自分泌方式调控通路活性。

Objective To explore the expression of PTCH protein in the Hedgehog pathway of suppressor gene and significance in methylation of non -small cell lung cancer （NSCLC） tissues. Methods To detect the methylation status of signal pathway correlated suppressor gene in NSCLC by using methyla- tion specific polymerases chain reaction （MS - PCR）. Results The specificity of Hedgehog pathway sup- prcssor gene are different in NSCLC, GAS gene is 82. 61%, HHIP gene is 69. 57%, PTCH gene is 100.00% , the differences have statistical significance （P 〈0. 01 ）. Conclusion HH pathway suppressor gcne may have different mechanisms in lung adenocarcinoma and squamous cell carcinoma. In adenocarci- noma, it may regulate activity of PTCH through paracrining, while in squamous carcinoma it may be regu- late activity of pathways by autocrining manner.