程序性死亡受体-1在人食管鳞状细胞癌组织中的表达及其与预后的关系

Programmed death 1 expression on tumor tissues correlates with prognosis in esophageal squamous cell carcinoma patients

目的观察食管鳞状细胞癌（ESCC）患者癌组织中程序性死亡受体-1（PD-1）表达，探讨其与患者预后关系。方法225例ESCC组织蜡块分组为短期生存（ss）、超长期生存（ULS）、癌旁对照组织芯片。免疫组织化学（IHC）法检测组织中PD-1表达；采用t检验、方差分析确定差异显著性；Kaplan—Meier分析、Cox多因素回归模型分析PD-1蛋白表达与患者预后关系。结果不同组别患者PD-1蛋白表达比较差异有统计学意义[PD-1SS组=45，PD-1ULS组=27，PD-1对照组=10，X2=61．307，P〈0．05]，且与肿瘤浸润深度（X2=9．442，P〈0．01）、淋巴结有无转移（r=8．599，P〈0．05）有关；生存分析显示PD-1阴性表达者预后好于阳性表达者（PD-1（-）=41，PD-1（＋）=47，PD-1（。）=25，X2=14．155，P〈0．01）；但其不是患者预后独立影响因素[相对危险度（RR）=1．44；95％可信区间（CI）：1．01—2．06；P〉0．05]。结论PD．1蛋白表达与ESCC患者肿瘤浸润深度、淋巴结有无转移及预后有关。

Objective To determine programmed death 1 （PD - 1 ） expression on tumor tissues correlates with prognosis in esophageal squamous cell carcinoma （ESCC） patients. Methods lmmunohis- tochemisty （IHC） was performed to detect the expression of PD - 1 in ESCC tumor tissues and health con- trois. The ehi - square test was applied for comparisons among different groups. Kaplan - Meier （ K - M） analysis and Cox multivariate survival analysis assessed the associations of PD - 1 expression with ESCC pa- tient prognosis. Results PD - 1 expressions in shorter survival （SS） group, ultra - longer survival （ULS） group and control group had a significantly difference [ PD - 1 ss group = 45, PD - 10LS group = 27, PD - 1 control group = 10, X2 = 61. 307, P 〈 0.05 ]. The presence of PD - l was related with tumor invasion （X2 = 9. 442, P 〈 0. 01 ） and lymph node metastasis （X2 = 8. 599, P 〈 0. 05 ）. K - M survival analysis showed that patients for PD - 1 negative expression was longer survival than those of positive ultra - positire expression [ PD - 1 （nesative） = 41, PD - 1 （positive） ： 47, PD - 1 （ultra-positive） ： 25, X2 = 14. 155, P 〈 0. O1 ]. Multivariate analysis demonstrated that PD - 1 was not an independent prognostic factor for ESCC patients survival [ relative risk （RR） = 1.44 ; 95% confidence interval （CI） ： 1.01 - 2. 06 ; P 〉 0. 05 ]. Condu- sion The expression of PD - 1 was correlated with tumor invasion and lymph node metastasis, the worst survival with the best positively expression.