TET家族蛋白在胃癌组织中的表达及其临床意义

The expression of ten - eleven transiocation family proteins in gastric cancer and its clinical significance

目的观察胃癌组织和癌旁组织中的TET1、TET2、TET3蛋白表达水平，探讨三者相关性及临床意义。方法通过免疫组织化学方法检测100例胃癌患者的肿瘤组织和癌旁组织中TET1、TET2、TET3蛋白表达，分析其与临床病理特征和预后的相关性。结果胃癌组织中TET1、TET2表达水平为2．45±0．29、4．66±0．25，显著低于癌旁组织（6．04±0．10．5．89±0．09），而TET3表达水平（7．84±0．17）显著高于癌旁组织（6．69±0．10），差异有统计学意义（P〈0．01）。TET1、TET2、TET3三者在胃癌组织中的表达无明显相关。TET1表达与幽门螺杆菌（Hp）感染、肿瘤大小、神经侵犯、淋巴转移、TNM分期显著相关（P〈0．05）。生存分析显示TET1下调的患者总生存期显著减短，多因素分析显示TET1表达是胃癌预后独立的风险因素。结论TET1下调与胃癌转移、临床分期显著相关，并提示预后不良。

Objective To measure the protein expression of ten - eleven translocation 1 （ TET1 ）, TET2, and TET3 genes in gastric cancer （GC） tissues and normal tissues, analyze the relationship be- tween the expression level of TET genes and clinicopathological parameters, and determine the prognostic potential of TET protein in GC. Methods This study included 100 patients with GC. The expression levels of TET1, TET2, and TET3 proteins in tissues were detected by immunohistochemistry. The association of TET protein expression with clinicopathological characteristics was analyzed by the method of Person＇ s chi - square test, and survival was calculated by log - rank test and Kaplan - Meier method. Cox regression model was used to perform multivariate analysis. Results The expression of TETI （2. 45 ± 0. 29） and TET2 （4. 66 ±0. 25） in GC tissue was significantly lower than in normal tissue （6. 04 ±0. 10, and 5.89 ±0. 09）, and TET3 expression level （7. 84±0. 17） in GC tissue was higher than in normal tissue （6. 69 ± 0. 10 ,P 〈 0. 01 ）. The expression of TET1 in GC was related to Hp infection, tumor size, neural invasion, lymph node metastasis, and TNM stage significantly （ P 〈 0. 05 ）. In addition, the expression of TET3 in GC was correlated to neural invasion, vascular invasion, depth of invasion and TNM stage （ P 〈 0. 05 ）. Moreover, TET1 expression in GC tissue was associated with overall survival of patients with GC. Multiva- riate analysis showed that TET1 expression in GC was an independent impact factor for prognosis of GC pa- tients [ （ hazard ratio （HR） ： 0. 036, 95 % confidence interval （CI） ： 0. 002 - 0. 681,P 〈 0. 05 ]. Conclu- sion TET1 is down -regulated significantly in GC and TET1 expression correlates to metastasis and over- all survival, suggesting that TET1 may be a potential marker for prognostic evaluation.