高效液相色谱法测定血清中茶碱和速尿

Determination of Theophylline and Furosemide in Serum by High—performance Liquid Chromatography

本文研究了反相高效液相色谱法分离和测定茶碱和速尿的诸条件,提出了液相色谱法测定和萃取血清中茶碱和速尿的新方法;该法简单、灵敏、精确。线性范围:茶碱为1—70μg/ml,速尿为0.5—100μg/ml;血清中茶碱和速尿浓度的日内与日间变异不大于5.5%。

The cited components in serum were separated and determined on a CLC—ODS column (15cm×6.0mm) with methanol—water (35:65, V/V) containing 0.02M Li_2SO_4 as a mobile (0.5ml/min). Detection was made at 254 nm. The calibration graphs were rectilinear from 1.0—70 ppm for theo phylline and 0.5—100ppm for furosemide. Detection limits were 0.1 ng for theophylline and 0.5 ng for furosemide. All inter— and intra—day coefficients of variation were below 5.5%.