发酵法制备L-鸟氨酸苯乙酸盐的结晶工艺

Preparation of crystalline L-ornithine phenylacetate from L-ornithine fermentation broth

L-鸟氨酸发酵液经膜分离、离子交换处理,再加入苯乙酸进行反应,制备结晶型L-鸟氨酸苯乙酸盐(OP)。采用单因素试验考察了晶种加入时机、晶种投加量、乙醇滴加速率及搅拌速率等因素对所得平均粒径及纯度的影响。确定起始质量浓度为800 g/L结晶液的较优结晶工艺条件为:乙醇与起始结晶液体积比为0.4时投加4wt%晶种,乙醇滴加速率0.8 m L/min,搅拌速率200 r/min。所得OP晶体平均粒径为614.7μm,纯度为98.3%,总收率为72%;将其X-射线粉末衍射图谱与文献图谱比对,确定所制备的晶体为OP晶体。

Herein crystalline L-ornithine phenylacetate was prepared by complexation of L-ornithine fermentation broth pretreated by membrane separation and ion exchange with benzene acetic acid. The influences of the adding time and amount of seed crystals, dropping speed of ethanol and stirring rate on the average particle size and purity of the crystalline were investigated by single factor test. The optimal crystallization conditions for mother liquid with a primary concentration of 800 g/L were as follows： 4wt% seed crystals were used when the volume ratio of anhydrous ethanol to initial crystal liquor was 0.4, together with a dropping rate of 0. 8 mL/min for ethanol and a stirring rate of 200 r/min. The mean diameter of the final product was 614.7 ixm and its purity was 98.3%. The total yield of the final product was 72%. The obtained product was identified by comparing its X-ray powder diffraction pattern with the literature.