摘要
作为一种液液萃取技术,双水相已广泛应用于多种生物制品的提取纯化,如蛋白质、遗传物质、小分子物质、细胞及细胞器等。其主要技术优势:易于连续化操作、操作条件温和、易于扩大化生产,文中主要介绍双水相法提取生物制品,双水相与其他新技术相结合的应用及双水相技术面临的问题与发展趋势。
Aqueous Two-Phase Systems (ATPS) is a liquid-liquid extraction technique that has been widely used to extract and purify a variety of biological products, including proteins, genetic material, low molecular weight products, cells and cell organelles. The main advantages of this technique include continuous processing, mild operating conditions and feasible to scale up. In this review, the application of ATPS for the extraction of biological products and ATPS combined with other new technology are highlighted. Problems and trends of the application of ATPS are discussed.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第7期268-273,共6页
Food and Fermentation Industries
基金
山东省高等学校科技计划项目
J11LC12
关键词
双水相萃取
生物制品
应用
aqueous two-phase systems extraction, biological products, application