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全肠外营养相关肠黏膜免疫屏障损伤小鼠模型的建立 被引量:6

Total parenteral nutrition associated intestinal immunity barrier impairment in a mouse model
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摘要 目的:建立一种稳定有效的小鼠模型,为进一步研究全肠外营养(TPN)相关肠黏膜屏障损伤的机制和干预措施。方法:将22只小鼠随机分为对照组(n=10)和TPN组(n=12)。小鼠经颈内静脉置管后,对照组给予正常饮食,用微量泵持续输注等渗盐水;TPN组给予禁食后用微量泵持续输注PN液。5 d后比较两组小鼠生存率、肠道菌群易位情况、肠道杯状细胞数量以及潘氏细胞变化。结果:两组小鼠的生存率无显著性差异。TPN组肠道菌群淋巴结易位率增加(6/10 vs 1/10,P<0.05),杯状细胞数量减少[(61.6±6.5)个vs(33.0±7.8)个,P<0.01)],隐窝处潘氏细胞内抗菌多肽颗粒明显减少,溶菌酶和黏蛋白2(MUC2)含量显著降低。结论:TPN组小鼠能作为后期研究TPN诱导肠黏膜免疫屏障受损的模型,用于患病机制、药物治疗的观察和研究。 Objective: This study was to establish a stable and effective mouse model for further investigation in total parenteral nutrition (TPN) associated intestinal barrier impairment. Methods : 22 mice were randomly arranged into control group (n = 10 ) or TPN group (n = 12 ). After the central venous catheter was placed in the fight internal jugular, isotonic saline ( control group ) or parenteral nutrition( TPN group) was infused through the catheter. Survival rate, intestinal bacteria translocation, the change of goblet cells and Paneth cells were observed. Results: No difference was observed between the two groups in survival rate. TPN group had more bacteria-positive MLN (6/10 vs 1/10, P 〈 0.05) and decreased numbers of goblet cells (61.6 ±6.5 vs 33.0 ±7.8, P 〈 0.01 ). The antibacterial peptides decreased significantly in TPN group compared with control group. Conclusion: The mouse model is suitable for investigating the pathogenesis and treatment of total parenteral nutrition associated intestinal immunity barrier impairment.
出处 《肠外与肠内营养》 CAS 北大核心 2015年第4期222-225,230,共5页 Parenteral & Enteral Nutrition
关键词 全肠外营养 肠屏障损伤 黏膜免疫 Total parenteral nutrition Intestinal barrier impairment Immunity barrier
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