Pokemon和c—mye表达及临床意义在结直肠癌中的

Expressions of Pokemon and c-myc in colorectal cancer and their clinical significance

目的检测Pokemon和c．myc在结直肠癌、结直肠腺瘤和正常结直肠黏膜组织中的表达情况，研究结直肠癌组织中Pokemon和c—myc的表达与临床病理因素的关系。方法收集86例结直肠癌患者肿瘤组织标本，60例结直肠腺瘤组织标本和40例正常结直肠黏膜组织标本。采用免疫组织化学法检测组织中Pokemon和c．myc蛋白表达情况，反转录聚合酶链反应检测组织中Pokemon和c—myc基因表达水平，并分析其与结直肠癌临床病理因素的关系。结果结直肠癌组织中Pokemon和c—myc蛋白阳性表达率均明显高于结直肠腺瘤组织和正常结直肠黏膜组织[69．8％（60／86）比13．3％（8／60）和5．0％（2／40）、73．3％（63／86）比15．0％（9／60）和2．5％（1／40）]，结直肠癌组织中Pokemon和c—myc基因表达水平均明显高于结直肠腺瘤组织和正常结直肠黏膜组织（0．915±0．247比0．358±0．102和0．277±0．085、1．272±0．360比0．398±O．153和0．255±0．097），差异有统计学意义（P〈0．05）。结直肠癌组织中Pokemon和c—myc基因的表达水平与肿瘤浸润程度、肿瘤分化程度、淋巴结转移、远处转移和Dukes分期有关，差异有统计学意义（P〈0．05），但号l生别、年龄和肿瘤直径无关（P〉0．05）。结论Pokemon和c—myc在结直肠癌组织中高表达，且这种表达与结直肠癌临床病理特征有关，提示Pokemon和c—myc可作为结直肠癌患者临床治疗的评估指标。

Objective To investigate the expressions of Pokemon and c-myc in colorectal cancer, colorectal adenomas and normal colorectal mucosa tissues, and demonstrate its relationship with elinicopathological features and prognosis in patients with colorectal cancer. Methods The specimens were taken from colorectal cancer tissue of 86 patients, colorectal adenomas tissue of 60 patients, and 40 normal coloreetal mucosa tissue. The expressions of Pokemon and c-myc protein were detected by immunohistochemistry, and the expressions of Pokemon and c-myc gcne were detected by reverse transcription polymerase chain reaction （RT-PCR）. The correlation between the expressions of Pokemon and c-myc, and clinicopathological features was analyzed. Results The positive expression rates of Pokemon and c-myc protein in colorectal cancer tissue were significantly higher than those in colorectal adenomas tissue and normal colorectal mucosa tissue： 69.8% （60/86） vs. 13.3% （8/60） and 5.0% （2/40）, 73.3% （63/86） vs. 15.0% （9/60） and 2.5%（1/40）, and the expression levels of Pokemon and c-mye gene in colorectal cancer tissue were significantly higher than those in colorectal adenomas tissue and normal colorectal mucosa tissue： 0.915 ±0.247 vs. 0.358 ± 0.102 and 0.277 ± 0.085, 1.272 ±0.360 vs. 0.398 ±0.153 and 0.255 ± 0.097, there were statistical differences （P 〈 0.05）. The expression levels of Pokemon and c-myc gene in colorectal cancer tissue had a close correlation with the degree of infiltration, histological differentiation, lymph node metastasis, distant metastasis and Dukes stage, there were statistical differences （P 〈 0.05）, but had no correlation with the sex, age, and tumor diameter （P 〉 0.05）. Conclusions The expression levels of Pokemon and c-myc are higher in colorectal cancer tissue, and the expressions are related to the clinicopathological features of colorectal cancer. The expression levels of Pokemon and c-myc may predict the prognosis in patients with colorectal cancer.