表达猪瘟病毒E0-E2基因重组腺病毒疫苗在兔体上的免疫原性分析

Immunogenicity Analysis of Recombinant Adenoviruses Expressing E0-E2 Genes of Classical Swine Fever Virus Vaccine in Rabbits

研究表达猪瘟病毒E0-E2基因重组腺病毒疫苗(rAd-E0-E2)在兔体上的免疫原性,并确定其最小免疫保护剂量。将rAd-E0-E2按10倍梯度稀释为107.0 IFU、106.0 IFU、105.0 IFU,分别免疫接种家兔,14d后,用猪瘟脾淋苗对各组兔子进行攻毒。结果107.0 IFU组和猪瘟脾淋苗组均未出现定型热反应(0/4),脾重/体重指数比值分别为0.055%、0.05%,脾组织切片均未见充血、淤血,用RT-PCR和IFA方法均未检测到脾脏内有猪瘟脾淋苗病毒;106.0 IFU组3/4出现定型热反应,脾重/体重指数比值为0.074%,脾组织切片3/4可见充血、淤血,用RT-PCR和IFA方法可检测到3/4脾脏有猪瘟脾淋苗病毒;105.0 IFU组和对照组全部出现定型热反应(4/4),脾重/体重指数比值分别为0.099%和0.102%,脾组织切片充血、淤血严重,用RT-PCR和IFA方法均可检测到兔子脾脏内有猪瘟脾淋苗病毒。结果表明与对照组均不保护和猪瘟脾淋苗组全部保护相比,rAd-E0-E2在兔体的最小免疫保护剂量为107.0 IFU,本研究成功完成了rAd-E0-E2在兔体上的免疫原性分析。

Study on the immunogenicity of recombinant adenoviruses expressing E0-E2 genes of classical swine fever virus vaccine （rAd-E0-E2） in rabbits and determination of the minimum vaccination dose were conducted. Ten-fold dilution of rAd-E0-E2 into 107.0 IFU,106.0 IFUI105.0 IFU was made. Rabbits were vac- cinated by each dilution and challenged with CSF spleen vaccine virus 14 dpi. In group 107.0 IFU and group CSF spleen vaccine, there were no Stereotypia thermal response （0/4）,the mean value of spleen weight/ body weight in both groups were respectively 0. 055%, 0. 05%, the histopathologic examination of the spleens turned out without hyperemia or congestion. There was no CSF spleen vaccine virus detected by RT-PCR and IFA methods,In Group 105.0 IFU, 3/4 rabbits showed Stereotypia thermal response, the mean value of spleen weight/body weight was 0. 074 %, the histopathologic examination of the spleens showed 3/ 4 hyperemia or congestion. 3/4 CSF spleen vaccine virus is detected by RT-PCR and IFA methods; In Group 105.0 IFU and the control group, all appeared Stereotypia thermal response （4/4）, the mean values of spleen weight/body weight in both were respectively 0. 099 % ,0. 102%, the histopathologic examination of the spleens showed serious hyperemia and congestion,CSF spleen vaccine virus was detected by RT-PCR and IFA methods. Compared to the control group, which offers 0% protection and group CSF spleen vac- cine,which offers 100% protection, the minimum vaccination dose of rAd-E0-E2 to protect the rabbit is 107.0 IFU. This study successfully completed the immunogenicity analysis of rAd-E0-E2 and detemined the minimum vaccination dose in rabbits.