摘要
目的 :观察中药合剂调节梗阻性黄疸大鼠细胞免疫功能的作用。方法 :采用结扎 SD大鼠胆总管的方法建立梗阻性黄疸大鼠模型。随机将大鼠分为正常对照组 (A)、假手术组 (B)、梗阻性黄疸组 (C)、梗阻性黄疸加中药合剂治疗组 (D) ,每组各 10只 ,D组用中药合剂灌胃 ,A、B、C组予 0 .9% Na Cl溶液灌胃。用药 2周后于心脏采血 ,用免疫荧光法在流式细胞仪上测定 T细胞表型 CD3、CD4 、CD8值 ,酶联免疫吸附试验法测定血清白细胞介素 - 2 (IL- 2 )的浓度。结果 :C组 CD3、CD4 、CD8、IL- 2值明显下降 ,与 A组比较差异有非常显著性意义 (P <0 .0 1) ,CD4 / CD8比值亦下降 (P <0 .0 5 ) ;D组与 C组比较 ,CD3、CD4 、IL- 2值显著升高 (P <0 .0 1) ,CD8值亦升高 (P <0 .0 5 )。结论 :梗阻性黄疸时存在细胞免疫功能抑制 ;中药合剂具有增强梗阻性黄疸细胞免疫功能的作用。
Objective:To investigate the change of cellular immune function in obstructive jaundice and the therapeutic effect of TCM mixture.Methods: Adult male SD rats were divided into four groups randomly(n=10):normal control group(group A);pseudo-operation group(group B), model group induced by ligating the bile duct (group C); model treated by TCM mixture(group D). Rats in group D were given TCM mixture by gavage for two weeks while in group A,B and C ,rats were given 0.9% natrii chloride. Blood samples were collected from heart after two weeks' treatment. The expression of CD_3, CD_4 and CD_8 were measured with fluoroimmunoassay(FIA) on flow-cytometer. Serum interleukin-2 (IL-2) was measured by enzyme linked immunosorbent assay(ELISA).Results: There was no difference between group A and B in the expression of CD_3,CD_4,CD_8 and IL-2 (P> 0.05) while marked difference between group A and C (P< 0.01).The ratio of CD_4 to CD_8 dropped in group C(P< 0.05) compared with that of group A.CD_3,CD_4 and IL-2 were noticeably increased in group D compared with that of group C(P< 0.01).The case was the same for CD_8(P< 0.05).Conclusion:The cellular immune function was suppressed in obstructive jaundice.TCM mixture was helpful to increase cellular immune function in obstructive jaundice.
出处
《中国中西医结合消化杂志》
CAS
2002年第4期225-227,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion