胡杨大片段基因在拟南芥中的表达及耐盐性分析

The expression of Populus euphratica large fragment gene in Arabidopsis and salt tolerance analysis

以根癌农杆菌为介导,利用拟南芥作为受体植物进行胡杨基因大片段的转化。共将1 570个胡杨BIBAC克隆进行了拟南芥转化实验,其中利用抗生素平板筛选,有590个克隆得到了转基因拟南芥,利用PCR进行检测后得到结果跟卡那平板筛选后的结果吻合。对得到的转基因拟南芥进行不同浓度Na Cl筛选(150和175 mmol/L),共得到10个有耐盐表现的克隆;进一步验证试验证实,有两个株系具有稳定的耐盐表现,分离出T2纯合系。对于所获得的转化子进行播种观察得到了两个表型变化的突变体。本研究对于解析胡杨耐盐机理及挖掘胡杨耐盐基因资源具有重要意义。

Mediated with Agrobacterium tumefaciens,we took Arabidopsis thaliana as the receptor plant and transferred large gene fragments of Populus euphratica into it. Totally,1 570 BIBAC clones of P. euphratica were transferred in this research and 590 from these clones got the Arabidopsis thaliana transformants by screening on antibiotic containing plate. Then PCR identification screening proved the results from antibiotic screening. These transformants of A. thaliana were screened on the different salt stresses of Na Cl（ 150 and 175 mmol / L and 10 clones could express salt tolerance. In these clones,two among them showed the stable salt tolerance and homozygote were gotten in the further experiments. The phenotype change of 2mutant strains were found in seeding experiments. The research would be meaningful for salt-tolerant mechanism and genetic resources of P. euphratica.